RPN2/Ribophorin II 蛋白, Human (HEK293, His)

RPN2/riboprotein II is a key subunit of the oligosaccharyltransferase (OST) complex, which catalyzes the initial glycan transfer during co-translational protein N-glycosylation. This critical step occurs in the nascent polypeptide chain and involves the transfer of defined glycans from polyhydroxypyrophosphate to asparagine residues in the Asn-X-Ser/Thr motif. RPN2/Ribophorin II Protein, Human (HEK293, His) is the recombinant human-derived RPN2/Ribophorin II protein, expressed by HEK293 , with C-His labeled tag. The total length of RPN2/Ribophorin II Protein, Human (HEK293, His) is 518 a.a., with molecular weight of ~60 kDa.

RPN2/Ribophorin II 是寡糖转移酶 (OST) 复合物的一个亚基，在催化脂质载体多醇中确定的聚糖（真核生物中的 Glc(3)Man(9)GlcNAc(2)）的初始转移中发挥着至关重要的作用-焦磷酸连接到新生多肽链中 Asn-X-Ser/Thr 共有基序内的天冬酰胺残基。这一关键步骤标志着蛋白质 N-糖基化的启动，这是蛋白质合成过程中发生的共翻译过程。OST 复合体（RPN2/核糖蛋白 II 是其中的一个组成部分）与易位子复合体上的 Sec61 复合体结合，促进蛋白质穿过内质网 (ER) 膜的易位。OST 复合物中所有亚基的协同存在对于实现最大酶活性至关重要。从本质上讲，RPN2/Ribophorin II 参与蛋白质修饰，特别是蛋白质糖基化，强调了其在 N-糖基化初始阶段的基本作用，有助于细胞中糖蛋白的结构和功能多样性。

Human

HEK293

C-His

P04844/NP_002942.2 (L23-V540)

6185  [NCBI]

LTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSASGTYTLYLIIGDATLKNPILWNVADVVIKFPEEEAPSTVLSQNLFTPKQEIQHLFREPEKRPPTV

Approximately 60 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.