RSPO1/R-spondin-1 蛋白, Mouse (189aa, His)

RSPO1 activates the canonical Wnt signaling pathway by binding to LGR4-6 receptors, triggering increased expression of target genes. It inhibits ZNRF3, regulating the Wnt signaling pathway, and negatively regulates the TGF-beta pathway. RSPO1 also plays a role in ovary determination and interacts with various proteins including FZD8, LRP6, and WNT1. It forms complexes with RNF43, LGR5, and LGR6, and promotes membrane clearance of ZNRF3. RSPO1 binds heparin and interacts with LGR4, LGR5, and LGR6. RSPO1/R-spondin-1 Protein, Mouse (189aa, His) is the recombinant mouse-derived RSPO1/R-spondin-1 protein, expressed by E. coli, with N-6*His labeled tag. The total length of RSPO1/R-spondin-1 Protein, Mouse (189aa, His) is 189 a.a., with molecular weight of ~23 kDa.

RSPO1 或 R-spondin-1 蛋白通过充当 LGR4-6 受体的配体，充当经典 Wnt 信号通路的激活剂。与 LGR4-6 结合后，RSPO1 与磷酸化的 LRP6 和卷曲受体结合，这些受体被细胞外 Wnt 受体激活。这种关联触发了经典的 Wnt 信号通路，导致靶基因的表达增加。RSPO1 还通过抑制 ZNRF3（Wnt 信号传导途径的重要调节因子）在调节经典 Wnt/β-连环蛋白依赖性途径和非经典 Wnt 信号传导中发挥作用。此外，RSPO1 作为卷曲 FZD8 和 LRP6 的配体，可能对 TGF-β 途径产生负调节。它在卵巢决定中发挥重要作用，并通过与 KREM1 相互作用拮抗 DKK1/KREM1 介导的 LRP6 内化来调节 Wnt 信号传导。RSPO1 与 ZNRF3 相互作用，促进 ZNRF3 和 LGR4 之间的间接相互作用，并促进 ZNRF3 的膜清除。它还在由 RNF43、LGR5 和 RSPO1 组成的复合物中被识别。RSPO1 与 FZD8 和 LRP6 的胞外结构域相互作用，但不与它们形成三元复合物。此外，RSPO1 与 WNT1 相互作用，结合肝素，并与 LGR4、LGR5 和 LGR6 相互作用。

Measured by its ability to induce alkaline phosphatase production by MC3T3-E1. The ED₅₀ for this effect is 72.8 ng/mL, corresponding to a specific activity is 1.3736×10⁴ units/mg.

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  Measured by its ability to induce alkaline phosphatase production by MC3T3-E1. The ED₅₀ for this effect is 72.8 ng/mL, corresponding to a specific activity is 1.3736×10⁴ units/mg.

Mouse

E. coli

N-6*His

Q9Z132 (S21-G209)

192199  [NCBI]

SRGIKGKRQRRISAEGSQACAKGCELCSEVNGCLKCSPKLFILLERNDIRQVGVCLPSCPPGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCQEALYLHKGRCYPACPEGSTAANSTMECGSPAQCEMSEWSPWGPCSKKRKLCGFRKGSEERTRRVLHAPGGDHTTCSDTKETRKCTVRRTPCPEG

Approximately 23 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.