RUVBL2 蛋白, Human (His-SUMO)

The RUVBL2 protein has DNA-stimulated ATPase and DNA helicase activities and can hexamerize for ATP hydrolysis. As a member of the NuA4 complex, RUVBL2 contributes to histone acetylation, altering nucleosome-DNA interactions to activate genes. RUVBL2 Protein, Human (His-SUMO) is the recombinant human-derived RUVBL2 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.

RUVBL2 蛋白具有单链 DNA 刺激的 ATP 酶和 ATP 依赖性 DNA 解旋酶（5' 至 3'）活性，六聚化被认为对 ATP 水解至关重要，环状结构中的相邻亚基有助于 ATP 酶活性。作为 NuA4 组蛋白乙酰转移酶复合物的组成部分，RUVBL2 主要通过核小体组蛋白 H4 和 H2A 的乙酰化参与选定基因的转录激活。这种修饰可能会改变核小体-DNA 相互作用，并促进修饰组蛋白与其他正向调节转录的蛋白质相互作用。NuA4 复合物，包括 ATP 酶和解旋酶活性，至少部分是由 RUVBL1 和 RUVBL2 与 EP400 的结合所促成。当 NuA4 被招募到 DNA 损伤位点时，也可能在 DNA 修复中发挥直接作用。此外，RUVBL2 是 SWR1 样复合物的一个组成部分，负责从核小体去除组蛋白 H2A.Z/H2AZ1，并被提议作为染色质重塑 INO80 复合物的核心成分，该复合物表现出 DNA 和核小体激活的 ATP 酶活性并催化 ATP 依赖性核小体滑动。RUVBL2 在 MYC 致癌转化中发挥重要作用，还通过 LEF1/TCF1-CTNNB1 复合物调节转录激活。此外，它可能抑制 ATF2 的转录活性，并参与内质网 (ER) 相关降解 (ERAD) 途径，负向调节 ER 应激反应基因的表达。RUVBL2 也可能在调节 U5 snRNP 复合物的组成中发挥作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-SUMO;N-6*His

Q9Y230-1 (A2-S463)

10856  [NCBI]

ATVTATTKVPEIRDVTRIERIGAHSHIRGLGLDDALEPRQASQGMVGQLAARRAAGVVLEMIREGKIAGRAVLIAGQPGTGKTAIAMGMAQALGPDTPFTAIAGSEIFSLEMSKTEALTQAFRRSIGVRIKEETEIIEGEVVEIQIDRPATGTGSKVGKLTLKTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSPHGIPIDLLDRLLIVSTTPYSEKDTKQILRIRCEEEDVEMSEDAYTVLTRIGLETSLRYAIQLITAASLVCRKRKGTEVQVDDIKRVYSLFLDESRSTQYMKEYQDAFLFNELKGETMDTS

Approximately 67.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.