S100A4 蛋白, Mouse (His)

The S100A4 protein, a calcium-binding protein, regulates cellular processes including motility, angiogenesis, differentiation, apoptosis, and autophagy.It interacts with MYH9 to enhance cell motility and chemotaxis.It modulates TP53 to reduce protein levels and stimulates cytokine production.S100A4 forms homodimers and interacts with PPFIBP1, ANXA2, TP53, CCR5, CXCR3, and FCGR3A, inhibiting FCGR3A phosphorylation.S100A4 Protein, Mouse (His) is the recombinant mouse-derived S100A4 protein, expressed by E.coli , with C-6*His labeled tag.

S100A4 蛋白是一种钙结合蛋白，在多种细胞过程中发挥着至关重要的作用，包括运动、血管生成、细胞分化、细胞凋亡和自噬。它通过与非肌肉肌球蛋白重链 IIA/MYH9 相互作用，导致细丝解聚并增加可溶性肌球蛋白-IIA 水平，最终形成促进趋化性的稳定突起，从而增强细胞运动性和侵袭性。此外，它通过与细胞核内的 C 端反式激活结构域结合来调节 TP53 的促凋亡功能，从而降低 TP53 蛋白水平。在细胞外空间，S100A4 刺激 T 淋巴细胞产生细胞因子，例如粒细胞集落刺激因子和 CCL24，并与 PGLYRP1 形成趋化复合物，通过 CCR5 和 CXCR3 受体促进淋巴细胞迁移。它形成同二聚体并与 PPFIBP1、膜联蛋白 2/ANXA2、TP53、CCR5、CXCR3 和 FCGR3A 相互作用，与 FCGR3A 相互作用抑制 FCGR3A 的 PKC 依赖性磷酸化。

Measured in a cell proliferation assay using U87 cells. The ED₅₀ for this effect is 7.658 ng/mL, corresponding to a specific activity is 1.306×10⁵ units/mg.

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  Measured in a cell proliferation assay using U87 cells. The ED₅₀ for this effect is 7.658 ng/mL, corresponding to a specific activity is 1.306×10⁵ units/mg.

Mouse

E. coli

C-6*His

P07091 (M1-K101)

20198  [NCBI]

MARPLEEALDVIVSTFHKYSGKEGDKFKLNKTELKELLTRELPSFLGKRTDEAAFQKVMSNLDSNRDNEVDFQEYCVFLSCIAMMCNEFFEGCPDKEPRKK

Approximately 13.0 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.