S6K1/RPS6KB1 蛋白, Human (sf9, GST)

S6K1/RPS6KB1 is a kinase downstream of mTOR that regulates cellular processes critical for proliferation, growth, and cell cycle. It phosphorylates EIF4B, RPS6 and EEF2K and regulates protein synthesis by inhibiting the pro-apoptotic function of BAD, thereby enhancing cell survival. S6K1/RPS6KB1 Protein, Human (sf9, GST) is the recombinant human-derived S6K1/RPS6KB1 protein, expressed by Sf9 insect cells , with N-GST labeled tag.

S6K1/RPS6KB1 是一种丝氨酸/苏氨酸蛋白激酶，在 mTOR 信号下游响应生长因子和营养物质发挥作用，协调对增殖、细胞生长和细胞周期进程至关重要的细胞过程。通过 EIF4B、RPS6 和 EEF2K 的磷酸化，它调节蛋白质合成，通过抑制 BAD 的促凋亡功能来促进细胞存活。在营养耗尽的条件下，失活形式与 EIF3 翻译起始复合物结合，在有丝分裂刺激下，mTORC1 磷酸化导致解离和激活。S6K1 还通过磷酸化预起始复合物中的底物在翻译起始中发挥关键作用。此外，它还通过响应 IGF1 磷酸化 EEF2 激酶来控制翻译延伸。此外，S6K1 参与 mTORC2 和 mTORC1 的反馈调节，介导细胞存活并调节胰岛素抵抗。值得注意的是，它通过 EPRS 磷酸化参与脂肪酸摄取的调节和干扰素 γ 诱导的翻译抑制。这些多方面的作用凸显了 S6K1 在协调不同细胞对环境信号的反应中的重要性。

No Kinase Activity.

Human

Sf9 insect cells

N-GST

P23443-1 (M1-L525)

6198  [NCBI]

Approximately 96 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% Glycerol, 1 mM GSH. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.