SARS-CoV-2 Guanine-N7 methyltransferase 蛋白 (His)

The ORF1ab gene is a specifically expressed gene of SARS-CoV-2 and can be quickly and sensitively detected to indirectly indicate the level of SARS-CoV-2. The ORF1ab protein is associated with viral replication and transcription, inhibition of host translation machinery, and suppression of host immune responses. SARS-CoV-2 Guanine-N7 methyltransferase Protein (His) is the recombinant Virus-derived SARS-CoV-2 Guanine-N7 methyltransferase protein, expressed by E. coli , with N-6*His labeled tag. The total length of SARS-CoV-2 Guanine-N7 methyltransferase Protein (His) is 527 a.a., with molecular weight of ~60.0 kDa.

ORF1ab 基因是 SARS-CoV-2 的特异表达基因，能够被快速灵敏地检测而间接指示 SARS-CoV-2 水平。在新型 COVID-19 大流行的进化动态中，SARS-CoV-2 早期进化为至少三个系统发育群，其特征是辅助蛋白 ORF3a 和 ORF8 的特定残基的正选择。已知的五种人类 β 冠状病毒均具有四种主要结构蛋白（E、M、N 和 S）和由 ORF1a 和 ORF1b 共同编码的 16 种非结构蛋白（ORF1ab），这些蛋白参与病毒的致病性和感染性。其中三个基因（E、S 和 ORF1ab 基因）在人类 β 冠状病毒中具有强烈的正选择特征，影响位于重要功能蛋白结构域的密码子。ORF1ab 蛋白在抑制宿主翻译机制、病毒复制和转录以及抑制宿主免疫反应中发挥重要作用。目前已开发了基于双探针杂交的电化学发光（ECL）生物传感器，采用“磁性捕获探针-靶向核酸-Ru(bpy)32+标记信号探针”的检测模型，对 ORF1ab 靶基因进行检测。

Virus

E. coli

N-6*His

YP_009725309.1 (A1-Q527)

43740578  [NCBI]

AENVTGLFKDCSKVITGLHPTQAPTHLSVDTKFKTEGLCVDIPGIPKDMTYRRLISMMGFKMNYQVNGYPNMFITREEAIRHVRAWIGFDVEGCHATREAVGTNLPLQLGFSTGVNLVAVPTGYVDTPNNTDFSRVSAKPPPGDQFKHLIPLMYKGLPWNVVRIKIVQMLSDTLKNLSDRVVFVLWAHGFELTSMKYFVKIGPERTCCLCDRRATCFSTASDTYACWHHSIGFDYVYNPFMIDVQQWGFTGNLQSNHDLYCQVHGNAHVASCDAIMTRCLAVHECFVKRVDWTIEYPIIGDELKINAACRKVQHMVVKAALLADKFPVLHDIGNPKAIKCVPQADVEWKFYDAQPCSDKAYKIEELFYSYATHSDKFTDGVCLFWNCNVDRYPANSIVCRFDTRVLSNLNLPGCDGGSLYVNKHAFHTPAFDKSAFVNLKQLPFFYYSDSPCESHGKQVVSDIDYVPLKSATCITRCNLGGAVCRHHANEYRLYLDAYNMMISAGFSLWVYKQFDTYNLWNTFTRLQ

Approximately 60.0 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM PB, 100 mM NaCl, 2 mM DTT, 0.1% Triton X-100, 20% glycerol, pH 6.0.

<1 EU/μg, determined by LAL method

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.