SARS-CoV-2 NSP7-NSP8 Heterodimer 蛋白 (His)

The ORF1ab gene is a specifically expressed gene of SARS-CoV-2 and can be quickly and sensitively detected to indirectly indicate the level of SARS-CoV-2. The ORF1ab protein is associated with viral replication and transcription, inhibition of host translation machinery, and suppression of host immune responses. SARS-CoV-2 NSP7-NSP8 Heterodimer Protein (His) is a recombinant protein dimer complex containing Virus-derived SARS-CoV-2 NSP7-NSP8 Heterodimer protein, expressed by E. coli, with C-His labeled tag. SARS-CoV-2 NSP7-NSP8 Heterodimer Protein (His), has molecular weight of ~32.8 kDa.

ORF1ab基因是SARS-CoV-2的特异性表达基因，可以快速、灵敏地检测以间接指示SARS-CoV-2的水平。在新型冠状病毒肺炎 (COVID-19) 大流行的进化动力学中，SARS-CoV-2 早期进化成至少三个系统发育群，其特征是辅助蛋白 ORF3a 和 ORF8 的特定残基的正选择。已知的五种人类β冠状病毒均具有四种主要结构蛋白（E、M、N和S）和由ORF1a和ORF1b共同编码的16种非结构蛋白（ORF1ab），它们参与病毒的致病性。和传染性。其中三个基因（E、S 和 ORF1ab 基因）的特征是人类 β 冠状病毒中的强正选择，影响位于功能重要的蛋白质结构域中的密码子。ORF1ab蛋白在抑制宿主翻译机制、病毒复制和转录以及抑制宿主免疫反应方面发挥着重要作用。开发了一种基于双探针杂交的电化学发光（ECL）生物传感器，采用“磁性捕获探针靶向核酸-Ru(bpy)32+标记信号探针”的检测模型来检测ORF1ab靶基因。检测。

Virus

E. coli

C-His

YP_009725303 (S1-Q83)&YP_009725304 (A1-Q198)

43740578  [NCBI]&43740578  [NCBI]

Approximately 32.8 kDa

Greater than 95% as determined by Tris-Bis PAGE

溶液.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 100 mM Arginine, pH 8.2. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.