SARS-CoV-2 Plpro/Papain-like protease 蛋白 (His)

SARS-CoV-2 NSP3 (NSP3) is one of non-structure protein that binds to viral RNA, nucleocapsid protein, as well as other viral proteins, and contributes in polyprotein processing. NSP3 has also an important role in innate immune response antagonism and is responsible for release of NSP1, NSP2, and NSP3 from the N-terminal region of pp1a and pp1ab. SARS-CoV-2 Plpro/Papain-like protease Protein (His) is the recombinant Virus-derived SARS-CoV-2 Plpro/Papain-like protease protein, expressed by E. coli , with N-His labeled tag.

SARS-CoV-2 NSP3 (NSP3) 是一种非结构蛋白，可与病毒 RNA、核衣壳蛋白及其他病毒蛋白结合，参与多蛋白加工。NSP3 在先天免疫应答拮抗中也有重要作用，并负责从 pp1a 和 pp1ab 的 N 端释放NSP1、NSP2 和 NSP3。NSP3 在刺激病毒 mRNA 的翻译中起着重要作用，这些 mRNA 被加帽修饰但没有聚腺苷化，而是在 NSP3 识别的 3' 处保守序列 'GACC' 终止，NSP3 与宿主 PABPC1 竞争 EIF4G1 结合。NSP3 与宿主 EIF4G1 的相互作用稳定了 EIF4E-EIF4G1 的相互作用，从而促进了加帽 mRNA 翻译的启动^[1][2]。

Virus

E. coli

N-His

QVD65746.1 (E1564-V1880)

43740578  [NCBI]

Approximately 36.79 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris 500 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.