1. 重组蛋白
  2. Viral Proteins
  3. SARS-CoV-2 Proteins
  4. SARS-CoV-2 Spike Proteins
  5. SARS-CoV-2 S2 Protein
  6. SARS-CoV S2 蛋白 (sf9, His)

SARS-CoV S2 蛋白 (sf9, His)

目录号: HY-P73667
产品使用指南

SARS-CoV 刺突糖蛋白 2 (S2) 是 SARS-CoV 刺突糖蛋白的一个亚基,与 S1 和 S2' 并列。S2 (668-1255) 作为一类病毒融合蛋白介导病毒粒子与细胞膜的融合,在内吞过程中,S2 被裂解成 S2' (798-1255)。内化到宿主细胞内体诱导 S 糖蛋白构象改变,从而通过组织蛋白酶 CTSL 蛋白水解揭示 S2 融合肽。SARS-CoV S2 蛋白 (sf9, His) 是重组的 SARS-CoV S2 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。

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描述

SARS-CoV spike glycoprotein 2 (S2) is a subunit of SARS-CoV Spike glycoprotein, among with S1 and S2'. S2 (668-1255) mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein, during endocytosis, S2 is cleaved into S2' (798-1255). Internalization into host cell endosomes induces S glycoprotein conformational changes, potentially unmasking the fusion peptide of S2 through cathepsin CTSL proteolysis. SARS-CoV S2 Protein (sf9, His) is the recombinant Virus-derived SARS-CoV S2 protein, expressed by Sf9 insect cells , with C-His labeled tag.

研究背景

SARS-CoV 刺突糖蛋白 2 (S2) 是 SARS-CoV 刺突糖蛋白的一个亚基,与 S1 和 S2' 并列。S1 (14-667) 可以通过与宿主受体相互作用将病毒粒子附着在细胞膜上,引发感染。S2 (668-1255) 作为一类病毒融合蛋白介导病毒粒子与细胞膜的融合,在内吞过程中,S2 被裂解成 S2' (798-1255)。
S 蛋白通过与人类 ACE2 和 CLEC4M/DC-SIGNR 受体的相互作用,将病毒粒子附着在细胞膜上,从而协调病毒进入。附着后,内化到宿主细胞内体诱导 S 糖蛋白构象改变,从而通过组织蛋白酶 CTSL 蛋白水解揭示 S2 融合肽[1][2]

种属

Virus

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

NP_828851.1 (S668-P1195)

基因 ID

1489668  [NCBI]

蛋白结构
N-term
SARS-CoV S2 (S668-P1195)
Accession # NP_828851.1
His
C-term
同用名
Spike glycoprotein; S glycoprotein; Peplomer protein; S
分子量

Approximately 59.65 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SARS-CoV S2 Protein (sf9, His)
目录号:
HY-P73667
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