SCGN 蛋白, Human (His)

SCGN Protein is a calcium-sensor protein with a regulatory role in glucose metabolism and the secretion of several peptide hormones. Secretagogin (SCGN) is a three-domain hexa-EF-hand Ca²⁺-binding protein. SCGN is a Ca²⁺-dependent generic molecular chaperone involved in protein homeostasis with broad substrate specificity. The overexpression of SCGN efficiently prevents cells from heat shock and oxidative damage. SCGN enhances pancreatic insulin secretion and is a useful biomarker of endocrine tumors, stroke, and psychiatric conditions^[1][2][3]. SCGN Protein, Human (His) is the recombinant human-derived SCGN protein, expressed by E. coli , with N-His labeled tag. The total length of SCGN Protein, Human (His) is 275 a.a., with molecular weight of ~34 kDa.

Secretagogin (SCGN) 是神经内分泌细胞的生物标志物，是位于染色体 6p22.3-p22.1 上的 SCGN 基因的基因产物。SCGN 是一种在神经内分泌细胞中高表达的钙结合蛋白，并且推测有 6 个 EF 手钙结合蛋白参与传递钙信号以控制细胞增殖。SCGN 增强胰腺胰岛素分泌，是内分泌肿瘤、中风和精神疾病的有用生物标志物。SCGN 还在阿尔茨海默病等神经退行性疾病中发挥神经保护作用。此外，RIN-5F胰岛素瘤细胞克隆在SCGN过度表达后表现出细胞生长迟缓，表明它们通过Ca2+信号调节参与生长控制和分化或抑制细胞复制^[1]。
细胞外 SCGN 很容易以能量依赖的方式内化到 C2C12 细胞中。SCGN 通过网格蛋白介导的内吞作用内化，随后 SCGN 大部分定位于细胞质中。外源 SCGN 治疗可诱导 C2C12 细胞中的整体蛋白质组重编程^[2]。
SCGN 主要在胰腺 β 细胞、大脑的某些部分以及神经内分泌组织中表达。SCGN 的表达在多种疾病中发生改变，例如糖尿病、癌症和神经退行性疾病。在存在 Ca²⁺ 的情况下，SCGN 可有效防止体外多种模型蛋白的聚集。Ca²⁺ 通过多态中间体诱导封闭紧凑的 apo-SCGN 构象转化为开放扩展的 Holo-SCGN 构象，这与 SCGN 伴侣活性的增强一致^[3]。

Human

E. coli

N-His

O76038 (D2-P276)

10590  [NCBI]

DSSREPTLGRLDAAGFWQVWQRFDADEKGYIEEKELDAFFLHMLMKLGTDDTVMKANLHKVKQQFMTTQDASKDGRIRMKELAGMFLSEDENFLLLFRRENPLDSSVEFMQIWRKYDADSSGFISAAELRNFLRDLFLHHKKAISEAKLEEYTGTMMKIFDRNKDGRLDLNDLARILALQENFLLQFKMDACSTEERKRDFEKIFAYYDVSKTGALEGPEVDGFVKDMMELVQPSISGVDLDKFREILLRHCDVNKDGKIQKSELALCLGLKINP

Approximately 34 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.