Semaphorin-5A/SEMA5A 蛋白, Mouse (HEK293, His)

Semaphorin-5A (SEMA5A) protein lacks essential conserved residue(s) for propagating feature annotation. Semaphorin-5A/SEMA5A Protein, Mouse (HEK293, His) is the recombinant mouse-derived Semaphorin-5A/SEMA5A protein, expressed by HEK293 , with C-His labeled tag. The total length of Semaphorin-5A/SEMA5A Protein, Mouse (HEK293, His) is 743 a.a., with molecular weight of ~95-110 kDa.

Semaphorin-5A (SEMA5A) 蛋白质缺乏传播特征注释所需的保守残基。

Measured by the ability of the immobilized protein to support the adhesion of PANC-1 human pancreatic cancer cells. The ED₅₀ for this effect is 1.226 μg/mL, corresponding to a specific activity is 815.661 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of PANC-1 human pancreatic cancer cells. The ED₅₀ for this effect is 1.226 μg/mL, corresponding to a specific activity is 815.661 units/mg.

Mouse

HEK293

C-His

Q3UPZ0 (E23-T765)

20356  [NCBI]

ETQDPAKCQRAEHPVVSYKEIGPWLREFRAENAVDFSRLTFDPGQKELVVGARNYLFRLELEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVCTIRSLSNLTEIHDQISGMARCPYSPQHNSTALLTASGELYAATAMDFPGRDPAIYRSLGTLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQGTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTMDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHLAVDVVQGRETLVHIIYLATDYGTIKKVRAPLSQSSGSCLLEEIELFPERRSEPIRSLQILHSQSVLFVGLQEHVAKIPLKRCHFHQTRSACIGAQDPYCGWDAVMKKCTSLEESLSMTQWDQSIPTCPTRNLTVDGSFGPWSPWTPCTHTDGTAVGSCLCRSRSCDSPAPQCGGWQCEGPRMEITNCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNACPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCST

Approximately 95-110 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.