Serpin D1 蛋白, Human (HEK293, His)

Serpin D1 protein is a thrombin inhibitor activated by glycosaminoglycans such as heparin or dermatan sulfate. It replaces antithrombin III and becomes the main thrombin inhibitor in the presence of dermatan sulfate. In addition to thrombin inhibition, serpin D1 also has glycosaminoglycan-independent chymotrypsin inhibitory activity. Serpin D1 Protein, Human (HEK293, His) is the recombinant human-derived Serpin D1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Serpin D1 Protein, Human (HEK293, His) is 480 a.a., with molecular weight of ~75-80 kDa.

Serpin D1 蛋白作为凝血酶抑制剂发挥作用，其活性由肝素或硫酸皮肤素等糖胺聚糖激活。在硫酸皮肤素存在下，HC-II 成为主要的凝血酶抑制剂，取代抗凝血酶 III (AT-III)。除了在凝血酶抑制中的作用外，丝氨酸蛋白酶抑制剂 D1 还具有独立于糖胺聚糖的胰凝乳蛋白酶抑制活性。值得注意的是，HC-II N 末端的肽对单核细胞和中性粒细胞均具有趋化活性。这种多方面的功能凸显了 Serpin D1 在调节蛋白酶活性和参与免疫反应中的多种作用，强调了其在维持止血和调节炎症过程中的重要性。

Measured by its ability to inhibit Thrombin cleavage of a peptide substrate, Boc-VPR-AMC. The IC₅₀ value is 0.344 nM, as measured under the described conditions.

Human

HEK293

C-His

P05546 (G20-S499)

3053  [NCBI]

GSKGPLDQLEKGGETAQSADPQWEQLNNKNLSMPLLPADFHKENTVTNDWIPEGEEDDDYLDLEKIFSEDDDYIDIVDSLSVSPTDSDVSAGNILQLFHGKSRIQRLNILNAKFAFNLYRVLKDQVNTFDNIFIAPVGISTAMGMISLGLKGETHEQVHSILHFKDFVNASSKYEITTIHNLFRKLTHRLFRRNFGYTLRSVNDLYIQKQFPILLDFKTKVREYYFAEAQIADFSDPAFISKTNNHIMKLTKGLIKDALENIDPATQMMILNCIYFKGSWVNKFPVEMTHNHNFRLNEREVVKVSMMQTKGNFLAANDQELDCDILQLEYVGGISMLIVVPHKMSGMKTLEAQLTPRVVERWQKSMTNRTREVLLPKFKLEKNYNLVESLKLMGIRMLFDKNGNMAGISDQRIAIDLFKHQGTITVNEEGTQATTVTTVGFMPLSTQVRFTVDRPFLFLIYEHRTSCLLFMGRVANPSRS

Approximately 75-80 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.