Serpin A8/Angiotensinogen 蛋白, Mouse (HEK293, His)

Serpin A8 (or angiotensinogen) is an important regulator in the renin-angiotensin system, fine-tuning blood pressure and electrolyte balance. This multifunctional protein acts as a potent vasoconstrictor, affecting vascular smooth muscle and affecting cardiac function via the sympathetic nervous system. Serpin A8/Angiotensinogen Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serpin A8/Angiotensinogen protein, expressed by HEK293 , with C-His labeled tag. The total length of Serpin A8/Angiotensinogen Protein, Mouse (HEK293, His) is 453 a.a., with molecular weight of ~58 kDa.

Serpin A8，俗称血管紧张素原，是肾素-血管紧张素系统（RAS）复杂框架中不可或缺的组成部分，在血压、体液和电解质稳态的精确调节中发挥着关键作用。这种多方面的蛋白质在各个生理领域发挥其影响，作为有效的血管收缩剂直接影响血管平滑肌。它的影响范围延伸到心血管系统，通过与交感神经系统的相互作用影响心脏收缩力和心率。此外，血管紧张素原通过刺激肾上腺皮质中的肾小球带细胞合成和释放醛固酮来调节钠和水的吸收，从而协调肾功能的动态变化。这些不同的作用是通过其与血管紧张素受体 AGTR1 和 AGTR2 的熟练结合来执行的。除了在 RAS 中的核心作用之外，血管紧张素原还参与其他相互作用，例如它与 DEAR/FBXW7-AS1 受体的结合，进一步扩大了其在复杂的细胞信号传导网络中的参与。

Measured in a cell proliferation assay using HT-29 human coloncancer cells. The ED ₅₀ for this effect is 41.71 ng/mL, corresponding to a specific activity is 2.398×10⁴ Unit/mg.

Mouse

HEK293

C-His

P11859 (D25-V477)

11606  [NCBI]

DRVYIHPFHLLYHNKSTCAQLENPSVETLPESTFEPVPIQAKTSPVNEKTLHDQLVLAAEKLEDEDRKRAAQVAMIANFVGFRMYKMLNEAGSGASGAILSPPALFGTLVSFYLGSLDPTASQLQTLLDVPVKEGDCTSRLDGHKVLAALRAVQGLLVTQGGSSSQTPLLQSIMVGLFTAPGFRLKHSFVQSLALFTPALFPRSLDLSTDPVLATEKINRFIKAVTGWKMNLPLEGVSTDSTLLFNTYVHFQGTMRGFSQLPGVHEFWVDNSISVSVPMISGTGNFQHWSDAQNNFSVTCVPLGERATLLLIQPHCTSDLDRVEALIFRNDLLTWIENPPPRAIRLTLPQLEIRGSYNLQDLLAEDKLPTLLGAEANLSNIGDTNPRVGEVLNSILLELKAGEEEQPTTSVQQPGSPEALDVTLSSPFLFAIYEQDSGTLHFLGRVNNPQSVV

Approximately 50-70 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.