Serum Albumin/ALB 蛋白, Human (Biotinylated, HEK293, His-Avi)

Serum Albumin/ALB Protein has versatile binding capabilities, binding water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin, and drugs. Its primary role involves regulating blood colloidal osmotic pressure for homeostasis. ALB acts as a major transporter for zinc, binding 80% of plasma zinc, and 45% of circulating calcium and magnesium. It interacts with the bacterial siderophore enterobactin, inhibiting E. coli's iron uptake. ALB also engages with FCGRT, regulating homeostasis, and forms a covalently-linked complex with chromophore-bound alpha-1-microglobulin, highlighting diverse molecular interactions and maintaining functionality in fatty acid binding. Serum Albumin/ALB Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Serum Albumin/ALB protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of Serum Albumin/ALB Protein, Human (Biotinylated, HEK293, His-Avi) is 585 a.a., with molecular weight of 69-70 kDa.

血清白蛋白/ALB 蛋白通过与水、Ca(2+)、Na(+)、K(+)、脂肪酸、激素、胆红素和药物结合而发挥多方面的作用，表明其具有多种结合能力。其主要功能可能围绕血液胶体渗透压的调节，强调其在维持血液稳态中的关键作用。值得注意的是，ALB 是锌的主要转运蛋白，结合约 80% 的血浆锌，同时也是钙和镁的重要转运蛋白，结合血浆中约 45% 的循环钙和镁。潜在钙结合位点的存在表明其参与钙转运，具有明显的亲和力顺序：锌>钙>镁。此外，ALB 与细菌铁载体肠杆菌素表现出特殊的相互作用，抑制肠杆菌素介导的大肠杆菌对铁的吸收，并可能限制肠道细菌对铁的利用。此外，ALB 与 FCGRT 相互作用，调节其稳态，并与血浆中发色团结合的 α-1-微球蛋白形成共价连接的复合物，突出其在分子相互作用中的多种作用并维持其在脂肪酸结合中的功能。

Biotinylated Human Serum Albumin, His-Avi Tag immobilized on CM5 Chip can bind Human FcRn, His Tag with an affinity constant of 1.84 μM as determined in SPR assay (Biacore T200).

Human

HEK293

C-Avi;C-His

P02768 (D25-L609)

213  [NCBI]

DAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL

69-70 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.