Serum Albumin/ALB 蛋白, Mouse (HEK293, His)

Serum Albumin/ALB Protein binds to various substances such as water, calcium, sodium, potassium, fatty acids, hormones, bilirubin, and drugs. Its main function is to regulate blood osmotic pressure and transport zinc, calcium, and magnesium. ALB may interact with both zinc and calcium, potentially influencing their transport. It inhibits the uptake of iron by E.coli through enterobactin but not aerobactin. ALB also interacts with FCGRT and TASOR for homeostasis. It forms a complex with alpha-1-microglobulin without affecting fatty acid binding. Serum Albumin/ALB Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serum Albumin/ALB protein, expressed by HEK293, with C-His labeled tag. The total length of Serum Albumin/ALB Protein, Mouse (HEK293, His) is 584 a.a., with molecular weight of ~70.19 kDa.

血清白蛋白/ALB 蛋白是一种多功能蛋白质，可与水、钙、钠、钾、脂肪酸、激素、胆红素和药物结合。其主要作用是调节血液的胶体渗透压。它是血浆中锌的主要转运蛋白，与大约 80% 的血浆锌结合。同样，它是钙和镁的主要转运蛋白，与血浆中大约 45% 的循环钙和镁结合。它可能具有两个以上的钙结合位点，并且还可以以非特异性方式结合钙。锌和钙之间的共享结合位点表明血液中这两种元素之间存在潜在的相互作用和运输机制。此外，ALB 与细菌铁载体肠杆菌素结合，抑制其促进大肠杆菌从铁转铁蛋白中摄取铁的能力。铁利用的这种限制可能会限制肠道细菌（例如大肠杆菌）的生长。然而，ALB 不会阻碍细菌铁载体需氧菌素促进的铁吸收。ALB 还与 FCGRT 相互作用，调节其自身的稳态，并与 TASOR 相互作用。在血浆中，它与发色团结合的 α-1-微球蛋白形成共价连接的复合物，但这种相互作用不会干扰脂肪酸与 ALB 的结合。

Mouse Serum Albumin, His Tag immobilized on CM5 Chip can bind Mouse FcRn, His Tag with an affinity constant of 54.53 nM as determined in SPR assay (Biacore T200).

Mouse

HEK293

C-His

P07724 (E25-A608)

11657  [NCBI]

EAHKSEIAHRYNDLGEQHFKGLVLIAFSQYLQKCSYDEHAKLVQEVTDFAKTCVADESAANCDKSLHTLFGDKLCAIPNLRENYGELADCCTKQEPERNECFLQHKDDNPSLPPFERPEAEAMCTSFKENPTTFMGHYLHEVARRHPYFYAPELLYYAEQYNEILTQCCAEADKESCLTPKLDGVKEKALVSSVRQRMKCSSMQKFGERAFKAWAVARLSQTFPNADFAEITKLATDLTKVNKECCHGDLLECADDRAELAKYMCENQATISSKLQTCCDKPLLKKAHCLSEVEHDTMPADLPAIAADFVEDQEVCKNYAEAKDVFLGTFLYEYSRRHPDYSVSLLLRLAKKYEATLEKCCAEANPPACYGTVLAEFQPLVEEPKNLVKTNCDLYEKLGEYGFQNAILVRYTQKAPQVSTPTLVEAARNLGRVGTKCCTLPEDQRLPCVEDYLSAILNRVCLLHEKTPVSEHVTKCCSGSLVERRPCFSALTVDETYVPKEFKAETFTFHSDICTLPEKEKQIKKQTALAELVKHKPKATAEQLKTVMDDFAQFLDTCCKAADKDTCFSTEGPNLVTRCKDALA

Approximately 70.19 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.