SHH 蛋白, Human

SHH, Human, as a recombinant protein, is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. SHH, Human, produced in E.Coli, is a single, non-glycosylated polypeptide chain. SHH plays an important role in prostate cancer progression.

音速刺猬（SHH）是刺猬基因家族的成员之一。SHH 信号在胚胎发育（包括肺）中起着至关重要的作用。SHH 对于发育过程中的模式形成很重要。SHH 转录受到包含多个增强子^[1][2]的长程调控机制的调节。

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is ≤ 0.3049 µg/mL, corresponding to a specific activity is ≥3.279×10³ units/mg.

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  Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.07853 µg/mL, corresponding to a specific activity is 1.27×10⁴ units/mg.

Human

E. coli

Tag Free

Q15465 (C24-G197)

6469  [NCBI]

CGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKISRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG

Approximately 20 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 100 mM NaCl, 1 mM DTT, pH 7.5 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wang XZ, et al. Sonic hedgehog (Shh) and CC chemokine ligand 2 signaling pathways in asthma. J Chin Med Assoc. 2019;82(5):343-350.  [Content Brief]

  [2]. Seo H, Amano T, Seki R, et al. Upstream Enhancer Elements of Shh Regulate Oral and Dental Patterning. J Dent Res. 2018;97(9):1055-1063.  [Content Brief]