1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. CARKL 蛋白, Human (HEK293, His)

CARKL 蛋白, Human (HEK293, His)

目录号: HY-P71308
产品使用指南

CARKL 蛋白作为巨噬细胞活化的调节剂,控制葡萄糖代谢并影响巨噬细胞的功能表型。它的调节作用强调了它在塑造细胞过程中的重要性,并有助于理解控制免疫反应和细胞稳态的分子机制。CARKL 蛋白, Human (HEK293, His) 是重组的 CARKL 蛋白,由 HEK293 表达,带有 C-6*His 标签。

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描述

CARKL Protein acts as a modulator of macrophage activation, controlling glucose metabolism and influencing the functional phenotype of macrophages. Its regulatory role underscores its significance in shaping cellular processes and contributes to understanding the molecular mechanisms governing immune responses and cellular homeostasis. CARKL Protein, Human (HEK293, His) is the recombinant human-derived CARKL protein, expressed by HEK293 , with C-6*His labeled tag.

研究背景

CARKL 蛋白作为巨噬细胞激活的调节剂,对葡萄糖代谢进行控制。它在细胞过程错综复杂的相互作用中的调节作用强调了它在协调巨噬细胞激活状态中的重要性。通过影响葡萄糖代谢,CARKL 可能在塑造巨噬细胞的功能表型方面发挥关键作用,有助于更广泛地了解控制免疫反应和细胞稳态的分子机制。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q9UHJ6 (M1-S478)

基因 ID
蛋白结构
N-term
CARKL (M1-S478)
Accession # Q9UHJ6
6*His
C-term
同用名
Sedoheptulokinase; SHK; Carbohydrate kinase-like protein; SHPK; CARKL
氨基酸序列

MAARPITLGIDLGTTSVKAALLRAAPDDPSGFAVLASCARAARAEAAVESAVAGPQGREQDVSRILQALHECLAALPRPQLRSVVGIGVSGQMHGVVFWKTGQGCEWTEGGITPVFEPRAVSHLVTWQDGRCSSEFLASLPQPKSHLSVATGFGCATIFWLLKYRPEFLKSYDAAGTIHDYVVAMLCGLPRPLMSDQNAASWGYFNTQSQSWNVETLRSSGFPVHLLPDIAEPGSVAGRTSHMWFEIPKGTQVGVALGDLQASVYSCMAQRTDAVLNISTSVQLAASMPSGFQPAQTPDPTAPVAYFPYFNRTYLGVAASLNGGNVLATFVHMLVQWMADLGLEVEESTVYSRMIQAAVQQRDTHLTITPTVLGERHLPDQLASVTRISSSDLSLGHVTRALCRGIVQNLHSMLPIQQLQEWGVERVMGSGSALSRNDVLKQEVQRAFPLPMSFGQDVDAAVGAALVMLRRHLNQKES

分子量

Approximately 53.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CARKL Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CARKL Protein, Human (HEK293, His)
目录号:
HY-P71308
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