SIGIRR 蛋白, Mouse (HEK293, His)

SIGIRR protein negatively regulates the Toll-like pathway and IL-1R pathway by inhibiting the recruitment of TLR4.SIGIRR disrupts Il1R1 and IL1RAP heterodimerization through its extracellular domain.SIGIRR Protein, Mouse (HEK293, His-hFc) is the recombinant mouse-derived SIGIRR protein, expressed by HEK293 , with C-hFc, C-His labeled tag.

SIGIRR 蛋白在 Toll 样和 IL-1R 受体信号通路中充当负调节因子，通过减弱受体近端信号成分向 TLR4 受体的募集来发挥其抑制作用，这可能是通过 TIR-TIR 结构域与 TLR4 的相互作用。此外，SIGIRR 通过其胞外结构域干扰 Il1R1 和 IL1RAP 的异二聚化。该蛋白与 IL1R1、IRAK1、TLR4、TLR5、TLR9 和 TRAF6 相互作用，并且在 IL-1 刺激后，发现它与 IL1R1、SIGIRR、MYD88、IRAK1 和 TRAF6 形成复合物。此外，在 LPC 刺激后，SIGIRR 是包含 TLR4、SIG1IR、MYD88、IRAK1 和 TRAF6 的复合体的一部分。它还与 PALM3 相互作用，表明它在调节炎症信号传导途径中具有多方面的作用。

Mouse

HEK293

C-His

Q9JLZ8 (M1-H117)

24058  [NCBI]

MAGVCDMAPNFLSPSEDQALGLALGREVALNCTAWVFSRPQCPQPSVQWLKDGLALGNGSHFSLHEDFWVSANFSEIVSSVLVLNLTNAEDYGTFTCSVWNVSSHSFTLWRAGPAGH

Approximately 20-40 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.