Siglec-10 蛋白, Human (HEK293, His)

Siglec-10 protein is thought to be a putative adhesion molecule that mediates sialic acid-dependent cell binding, preferentially selecting α-2,3- or α-2,6-linked sialic acid. Its sialic acid recognition site may be masked by cis interactions. Siglec-10 Protein, Human (HEK293, His) is the recombinant human-derived Siglec-10 protein, expressed by HEK293 , with C-His labeled tag.

Siglec-10 蛋白被认为是一种推定的粘附分子，在唾液酸依赖性细胞结合中发挥作用，显示出对 α-2,3- 或 α-2,6- 连接唾液酸的偏好。Siglec-10 的唾液酸识别位点可能由于与同一细胞表面上的唾液酸的顺式相互作用而被掩蔽。在免疫反应中，它似乎充当抑制性受体，诱导配体诱导的酪氨酸磷酸化并通过其 SH2 结构域招募细胞质磷酸酶，通过信号分子的去磷酸化来阻断信号转导。Siglec-10 依赖于 PTPN6/SHP-1，参与 B 细胞抗原受体信号传导的负向调节。与 CD24 结合，它可能参与选择性抑制对危险相关分子模式 (DAMP)（例如 HMGB1、HSP70 和 HSP90）的免疫反应。Siglec-10 与 CD24 协同调节自然杀伤 (NK) 细胞的免疫反应，并在控制自身免疫方面发挥作用。在 CD8-α(+) 树突状细胞启动适应性免疫反应期间，Siglec-10 通过损害 MHC I 类肽复合物的形成来抑制交叉呈递，从而招募 PTPN6/SHP-1 并促进吞噬体酸化。Siglec-10 与多种蛋白质相互作用，包括 PTPN6/SHP-1、NCF1、CD24、HMGB1、RIGI、CBL 和 PTPN11。

Immobilized Human Siglec-10 at 2 μg/mL (100 μL/well) can bind Anti-Siglec-10 Antibody, the ED₅₀ for this effectis 21.31 ng/mL.

Human

HEK293

C-His

AAI01726.1 (M17-N550)

89790  [NCBI]

MDGRFWIRVQESVMVPEGLCISVPCSFSYPRQDWTGSTPAYGYWFKAVTETTKGAPVATNHQSREVEMSTRGRFQLTGDPAKGNCSLVIRDAQMQDESQYFFRVERGSYVRYNFMNDGFFLKVTALTQKPDVYIPETLEPGQPVTVICVFNWAFEECPPPSFSWTGAALSSQGTKPTTSHFSVLSFTPRPQDHNTDLTCHVDFSRKGVSVQRTVRLRVAYAPRDLVISISRDNTPALEPQPQGNVPYLEAQKGQFLRLLCAADSQPPATLSWVLQNRVLSSSHPWGPRPLGLELPGVKAGDSGRYTCRAENRLGSQQRALDLSVQYPPENLRVMVSQANRTVLENLGNGTSLPVLEGQSLCLVCVTHSSPPARLSWTQRGQVLSPSQPSDPGVLELPRVQVEHEGEFTCHARHPLGSQHVSLSLSVHYSPKLLGPSCSWEAEGLHCSCSSQASPAPSLRWWLGEELLEGNSSQDSFEVTPSSAGPWANSSLSLHGGLSSGLRLRCEAWNVHGAQSGSILQLPDKKGLISTAFSN

Approximately 87.43 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 0.2 % CHAPS, 10 % trehalose, 0.1 M Arg, pH 8.0 or 20 mM Tris, 500 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.