1. 重组蛋白
  2. Receptor Proteins
  3. Siglec
  4. Siglec-8
  5. Siglec-8 蛋白, Human (HEK293, His-Avi)

Siglec-8 蛋白, Human (HEK293, His-Avi)

目录号: HY-P78513
产品使用指南

Siglec-8 蛋白是一种假定的粘附分子,介导唾液酸依赖性与红细胞的结合,更喜欢 α-2,3-连接的唾液酸并与 α-2,6-连接的唾液酸相互作用。其唾液酸识别位点可能通过与同一细胞表面的唾液酸顺式相互作用而被隐藏。Siglec-8 蛋白, Human (HEK293, His-Avi) 是重组的 Siglec-8 蛋白,由 HEK293 表达,带有 C-Avi, C-His 标签。

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描述

Siglec-8 protein is a putative adhesion molecule that mediates sialic acid-dependent binding to erythrocytes, preferring α-2,3-linked sialic acid and interacting with α-2,6-linked sialic acid . Its sialic acid recognition site may be hidden through cis interactions with sialic acid on the surface of the same cell. Siglec-8 Protein, Human (HEK293, His-Avi) is the recombinant human-derived Siglec-8 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

研究背景

Siglec-8 蛋白被认为是一种假定的粘附分子,充当唾液酸依赖性与红细胞结合的介体。它表现出优先结合 α-2,3-连接的唾液酸,并且还与 α-2,6-连接的唾液酸相互作用。值得注意的是,Siglec-8 的唾液酸识别位点可能由于与同一细胞表面上存在的唾液酸的顺式相互作用而被隐藏。此外,它能够同时识别以末端 N-乙酰神经氨酸(唾液酸)和底层 6-O-硫酸化半乳糖为特征的表位,并对 Gal-6-硫酸化唾液酸-Lewis X 聚糖表位具有特定的亲和力。

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

Q9P0M4/NP_037410.1 (M17-A363)

基因 ID
蛋白结构
N-term
Siglec-8 (M17-A363)
Accession # Q9P0M4/NP_037410.1
His-Avi
C-term
同用名
CDw329; MGC59785; SAF2; SAF2SAF-2; Siglec-8; SIGLEC8L; SIGLEC8
分子量

50-60 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Siglec-8 Protein, Human (HEK293, His-Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Siglec-8 Protein, Human (HEK293, His-Avi)
目录号:
HY-P78513
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