Siglec-E 蛋白, Mouse (HEK293, Fc)

Siglec-E Protein is a mouse orthologue of human Siglec-9 and functions as a key immunosuppressive checkpoint molecule. Siglec-E interacts with CD36 to inhibits downstream VAV signaling involved in modified LDL uptake, thereby delaying atherosclerosis. The endogenous inducible Siglec-E plays crucial anti-inflammatory and neuroprotective roles following ischemic stroke. Siglec-E Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Siglec-E protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Siglec-E Protein, Mouse (HEK293, Fc) is 336 a.a..

唾液酸结合 Ig 样凝集素 E (Siglec-E) 是人 Siglec-9 的小鼠直系同源物，是一种关键的免疫抑制检查点分子。Siglec-E 是一种唾液酸结合凝集素，主要在骨髓细胞表面表达，通过在与其唾液酸聚糖配体结合后招募含有 SH2 结构域的蛋白酪氨酸磷酸酶 SHP-1/2 来转导抑制信号。Siglec-E 与 CD36 相互作用，抑制参与修饰 LDL 摄取的下游 VAV 信号传导，进而延缓动脉粥样硬化。内源性诱导型 Siglec-E 在缺血性中风后发挥重要的抗炎和神经保护作用，因此可能是大脑炎症消退和自我修复的内在机制的基础。Siglec-E 是中性粒细胞向肺部募集和 β2 整合素依赖性信号传导的重要负调节因子，并抑制 CD11b β2 整合素依赖性信号传导^[1][2][3]。

Measured by its binding ability in a functional ELISA. Immobilized Mouse Siglec-E at 2 μg/mL (100 μL/well) can bind biotinylated Mouse Galectin-3. The ED₅₀ for this effect is 0.2911 μg/mL.

Mouse

HEK293

C-hFc

Q6PJ50/AAH23280 (Q20-F355)

83382

QNPQEGFTLNVERKVVVQEGLCVLVPCNFSYLKKRLTDWTDSDPVHGFWYREGTDRRKDSIVATNNPIRKAVKETRNRFFLLGEPWRNDCSLNIREIRKKDAGLYFFRLERGKTKYNYMWDKMTLVVTALTNTPQILLPETLEAGHPSNLTCSVPWDCGWTAPPIFSWTGTSVSFLSTNTTGSSVLTITPQPQDHGTNLTCQVTLPGTDVSTRMTIRLNVSYAPKNLTVTIYQGADSVSTILKNGSSLPISEGQSLRLICSTDSYPPANLSWSWDNLTLCPSKLSKPGLLELFPVHLKHGGVYTCQAQHALGSQHISLSLSPQSSATLSEMMMGTF

Approximately 78-100 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.5 or PBS, pH 6.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.