SIRP alpha/CD172a 蛋白, Human (G75A, HEK293, His)

The SIRP α V4/CD172a protein is an immunoglobulin-like cell surface receptor for CD47 that acts as a docking protein to facilitate the translocation of PTPN6, PTPN11, and other partners to the plasma membrane. It supports the adhesion of cerebellar neurons, promotes neurite growth, and promotes glial cell attachment. SIRP alpha/CD172a Protein, Human (G75A, HEK293, His) is the recombinant human-derived SIRP alpha/CD172a protein, expressed by HEK293 , with C-His labeled tag and G75A, , , , mutation.

SIRP α V4/CD172a 蛋白是一种 CD47 的免疫球蛋白样细胞表面受体，充当对接蛋白，促进 PTPN6、PTPN11 和其他结合配偶体从细胞质易位到质膜。该受体有助于多种细胞过程，包括支持小脑神经元的粘附、促进神经突生长和促进神经胶质细胞附着。SIRP α V4/CD172a 在突触发生和突触功能期间的细胞内信号传导中具有潜在作用，还参与由细胞粘附、生长因子或胰岛素触发的受体酪氨酸激酶偶联反应的负调节。此外，它介导吞噬作用、肥大细胞激活和树突细胞激活的负调节。值得注意的是，CD47 结合可防止未成熟树突细胞的成熟，并抑制成熟树突细胞产生细胞因子。SIRP alpha V4/CD172a 在抗病毒免疫中发挥着重要作用，通过减少病毒内化来限制新世界沙粒病毒感染。作为 THBS1 的受体，它在与 THBS1 相互作用后参与非吞噬细胞中的 ROS 信号传导，刺激 NADPH 氧化酶衍生的 ROS 产生。该受体参与各种蛋白质相互作用，包括与 PTPN11、GRB2、FGR、JAK2、SCAP1、SCAP2、FYB1、PTK2B 和 TRIM2 结合。

Immobilized Recombinant Human CD47 Protein (ECD,Fc Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human SIRP alpha / CD172a Protein (ECD, His Tag), the EC₅₀ is 280-850 ng/mL.

Human

HEK293

C-His

P78324-1 (E31-R370, G75A)

140885  [NCBI]

EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPARELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER

45-55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.