SKP1 蛋白, Human (Biotinylated, His-Avi)

The SKP1 protein is a component of the SCF ubiquitin ligase complex and targets cell cycle progression and signal transduction. SKP1 Protein, Human (Biotinylated, His-Avi) is the recombinant human-derived SKP1 protein, expressed by HEK293 , with C-Avi, N-His labeled tag. The total length of SKP1 Protein, Human (Biotinylated, His-Avi) is 162 a.a., with molecular weight of 23-28 kDa.

SKP1 是 SCF（SKP1-CUL1-F-box 蛋白）泛素连接酶复合物不可或缺的组成部分，在协调参与多种细胞过程（包括细胞周期进程、信号转导和转录）的蛋白质泛素化方面发挥着至关重要的作用。在 SCF 复合物中，SKP1 作为一个重要的接头，将 F-box 蛋白桥接至 CUL1，从而促进 SCF 复合物的底物识别功能。SCF 介导的泛素化的特异性取决于 F-box 蛋白独特的底物识别能力。SCF 复合体中的几种 F-box 蛋白，例如 BTRC、FBXW11、SKP2、FBXW7、FBXO32 等，参与指导针对参与 Wnt 信号传导、NF-kappa-B 激活、G1/S 的关键调节蛋白的泛素化事件转换调节和多种细胞途径。这种复杂的相互作用网络凸显了 SKP1 作为 SCF 泛素连接酶复合物中分子适配器的关键作用，通过泛素化控制蛋白质修饰的动态调节。

Human

HEK293

C-Avi;N-His

P63208 (P2-K163)

6500  [NCBI]

PSIKLQSSDGEIFEVDVEIAKQSVTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQWCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTCKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQWCEEK

23-28 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.