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  4. SLC14A1 蛋白, Human (Sf9, His, MBP, FLAG)

SLC14A1 蛋白, Human (Sf9, His, MBP, FLAG)

目录号: HY-P702009
产品使用指南

SLC14A1 蛋白在细胞过程中发挥着关键作用，介导尿素穿过红细胞和肾内髓质集合管膜的转运。它对于尿液浓缩机制至关重要，并调节红细胞中的水输送。SLC14A1 在尿素转运中具有双重作用，可维持细胞渗透平衡并有助于与尿液浓缩相关的生理过程，凸显了其重要性。SLC14A1 蛋白, Human (Sf9, His, MBP, FLAG) 是重组的 SLC14A1 蛋白，由 Sf9 insect cells 表达，带有 N-MBP, C-Flag, N-8*His 标签。SLC14A1 蛋白, Human (Sf9, His, MBP, FLAG) 全长 388 个氨基酸。

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100 μg   询价  

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描述

SLC14A1 Protein plays a pivotal role in cellular processes, mediating urea transport across erythrocyte and renal inner medullary collecting duct membranes. It is crucial for the urinary concentrating mechanism and regulates water transport in erythrocytes. With a dual role in urea transport, SLC14A1 maintains cellular osmotic balance and contributes to physiological processes associated with urinary concentration, highlighting its significance. SLC14A1 Protein, Human (Sf9, His, MBP, FLAG) is the recombinant human-derived SLC14A1 protein, expressed by Sf9 insect cells , with N-MBP, C-Flag, N-8*His labeled tag. The total length of SLC14A1 Protein, Human (Sf9, His, MBP, FLAG) is 388 a.a., .
研究背景

SLC14A1 蛋白在浓度梯度的驱动下介导尿素穿过红细胞细胞膜的转运，从而在细胞过程中发挥关键作用。此外，SLC14A1 通过介导尿素穿过肾内髓质集合管细胞膜的转运，在尿液浓缩机制中发挥着至关重要的作用。该功能对于红细胞中水运输的调节是不可或缺的。该蛋白质在尿素转运中的双重作用强调了其在维持细胞渗透平衡和促进与尿液浓度相关的生理过程中的重要性。
种属

Human
表达系统

Sf9 insect cells
标签

N-MBP;C-Flag;N-8*His
蛋白编号

Q13336 (E2-L389)
基因 ID

6563
蛋白结构
N-term
8*His-MBP
SLC14A1 (E2-L389)
Accession # Q13336
Flag
C-term
同用名
SLC14A1; Urea transporter 1; Solute carrier family 14 member 1; Urea transporter; erythrocyte
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

SLC14A1 Protein, Human (Sf9, His, MBP, FLAG) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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