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  4. SLC15A2 蛋白, Human (Sf9, His, Strep, FLAG)

SLC15A2 蛋白, Human (Sf9, His, Strep, FLAG)

目录号: HY-P702025
产品使用指南

SLC15A2蛋白是一种质子偶联氨基酸转运蛋白，偏爱二肽并转运含有2至4个氨基酸的寡肽。质子与肽的化学计量比为2:1或3:1，有助于肾脏吸收二肽和三肽。SLC15A2 蛋白, Human (Sf9, His, Strep, FLAG) 是重组的 SLC15A2 蛋白，由 Sf9 insect cells 表达，带有 N-Strep, C-Flag, N-8*His 标签。SLC15A2 蛋白, Human (Sf9, His, Strep, FLAG) 全长 728 个氨基酸。

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描述

The SLC15A2 protein is a proton-coupled amino acid transporter that prefers dipeptides and transports oligopeptides containing 2 to 4 amino acids. The stoichiometric ratio of protons to peptides is 2:1 or 3:1, which helps the kidneys absorb dipeptides and tripeptides. SLC15A2 Protein, Human (Sf9, His, Strep, FLAG) is the recombinant human-derived SLC15A2 protein, expressed by Sf9 insect cells , with N-Strep, C-Flag, N-8*His labeled tag. The total length of SLC15A2 Protein, Human (Sf9, His, Strep, FLAG) is 728 a.a., .
研究背景

SLC15A2 蛋白是一种质子偶联氨基酸转运蛋白，优先转运含有 2 至 4 个氨基酸的寡肽，对二肽具有显着的亲和力。其转运活性涉及 2:1 或 3:1 的质子与肽化学计量，促进肾脏内肾小球滤液中循环二肽和三肽的吸收。除了在肽转运中的作用之外，SLC15A2 通过转运各种物质而表现出多功能性，包括头孢羟氨苄等 β-内酰胺抗生素、其他抗病毒和抗癌药物、二肽样氨肽酶抑制剂贝他汀和肌肽。值得注意的是，它对先天免疫的参与延伸到促进 NOD 样受体 (NLR) 对微生物病原体的检测，并且它介导细菌肽聚糖跨细胞膜的运输，例如胞壁酰二肽 (MDP)，NOD2 的配体，特别是在巨噬细胞中。
种属

Human
表达系统

Sf9 insect cells
标签

N-Strep;C-Flag;N-8*His
蛋白编号

Q16348 (N2-L729)
基因 ID

6565
蛋白结构
N-term
8*His-Strep
SLC15A2 (N2-L729)
Accession # Q16348
Flag
C-term
同用名
SLC15A2; Solute carrier family 15 member 2; Kidney H(+)/peptide cotransporter; Oligopeptide transporter; kidney isoform; Peptide transporter 2
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

SLC15A2 Protein, Human (Sf9, His, Strep, FLAG) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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