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  3. SLC22A6 蛋白, Human (HEK293, His, MBP, FLAG)

SLC22A6 蛋白, Human (HEK293, His, MBP, FLAG)

目录号: HY-P704004
产品使用指南

SLC22A6 蛋白作为 Na(+) 独立的有机阴离子 (OA)/二羧酸反向转运蛋白,通过将 OA 从近端小管细胞转运到尿液中来促进肾脏消除。它转运辅酶(BH4、BH2、隔膜蝶呤)并测定血液生物蝶呤以及前列腺素、环核苷酸、色氨酸代谢物、尿毒症毒素(例如硫酸吲哚酚)和异生物质(例如赭曲霉毒素)的水平。SLC22A6 蛋白, Human (Sf9, His, MBP, FLAG) 是重组的 SLC22A6 蛋白,由 Sf9 insect cells 表达。

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描述

The SLC22A6 protein acts as a Na(+)-independent organic anion (OA)/dicarboxylic acid antiporter and promotes renal elimination by transporting OA from proximal tubule cells into the urine. It transports coenzymes (BH4, BH2, septopterin) and determines blood biopterin levels as well as prostaglandins, cyclic nucleotides, tryptophan metabolites, uremic toxins (e.g. indoxyl sulfate) and xenobiotics (e.g. ochratoxin). SLC22A6 Protein, Human (Sf9, His, MBP, FLAG) is the recombinant human-derived SLC22A6 protein, expressed by Sf9 insect cells .

研究背景

SLC22A6 蛋白作为次级主动转运蛋白发挥作用,作为 Na(+) 独立的有机阴离子 (OA)/二羧酸逆向转运蛋白。这种转运蛋白促进 OA 穿过近曲小管上皮细胞基底外侧的摄取,有助于肾脏将内源性 OA 从体循环中消除到尿液中。此外,SLC22A6 还参与将四氢生物蝶呤 (BH4)、二氢生物蝶呤 (BH2) 和墨蝶呤等辅酶转运至尿液,从而确定血液生物蝶呤的基线水平。它还在前列腺素、环核苷酸、神经活性色氨酸代谢物和潜在有毒化合物(如尿毒症毒素(例如硫酸吲哚酚、马尿酸)和异生物质(例如赭曲霉毒素))的运输中发挥作用。此外,SLC22A6 可能有助于通过肾脏有机阴离子分泌途径处理尿毒症毒素和异生物质,减少其不良毒理学作用。在睾丸中,它可能参与有机化合物穿过血睾屏障的运输。

种属

Human

表达系统

HEK293

标签

N-His;C-Flag;N-MBP

蛋白编号

Q4U2R8-1 (A2-L563)

基因 ID

9356

蛋白结构
N-term
8*His-MBP
SLC22A6 (A2-L563)
Accession # Q4U2R8-1
Flag
C-term
同用名
SLC22A6; Solute carrier family 22 member 6; Organic anion transporter 1; hOAT1; PAH transporter; hPAHT; Renal organic anion transporter 1; Hroat1
分子量

Approximately 105.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SLC22A6 Protein, Human (HEK293, His, MBP, FLAG) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SLC22A6 Protein, Human (HEK293, His, MBP, FLAG)
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HY-P704004
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