SLC31A1 蛋白, Human (Cell-Free, His-SUMO)

The SLC31A1 protein is an important uniporter that can transport copper(1+) from the extracellular space into the cytoplasm with remarkable specificity and deliver it directly to molecular chaperones such as ATOX1. This process tightly regulates intracellular copper(1+) levels and may be involved in intestinal copper absorption. SLC31A1 Protein, Human (Cell-Free, His-SUMO) is the recombinant human-derived SLC31A1 protein, expressed by E. coli Cell-free , with N-SUMO, N-6*His labeled tag. The total length of SLC31A1 Protein, Human (Cell-Free, His-SUMO) is 190 a.a., the molecular weight are 40 KDa (monomer), 80 KDa (dimer), whiel dimers are generally formed.

SLC31A1 是一种关键的单向转运蛋白，负责协调铜 (1+) 穿过质膜从细胞外空间转运至细胞质。这种运输具有显着的特异性，因为 SLC31A1 通过其 C 端结构域内的铜 (1+) 介导的瞬时相互作用，直接将铜 (1+) 传递到专门的分子伴侣，如 ATOX1。这一复杂的过程作为一种调节机制，严格控制细胞内铜 (1+) 水平。除了其典型作用之外，SLC31A1 通过潜在参与铜 (1+) 从顶膜的输入而表现出多功能性，表明在肠道铜吸收中发挥作用。该运输机制不依赖于钠，具有高亲和力驱动且可饱和。此外，SLC31A1 介导银 (1+) 的摄取，其参与含铂化疗药物的流入表明对治疗反应有潜在影响。体外研究证明 SLC31A1 能够将镉 (2+) 转运到细胞中。值得注意的是，SLC31A1 不仅充当铜转运蛋白，还充当氧化还原传感器，通过传递 VEGF 诱导的 ROS 信号促进内皮细胞的血管生成。该过程涉及 Cys-189 的磺酰化，导致 SLC31A1 和 KDR 之间形成二硫键，促进持续的 VEGFR2 信号传导。SLC31A1-KDR 复合物共内化到早期内体中，有助于延长 VEGFR2 信号传导。此外，SLC31A1 将铜 (1+) 动员出内体隔室，从而促进其从细胞中输出。这些不同的功能强调了 SLC31A1 在细胞铜稳态和信号通路中的多方面性质。

Measured by its binding ability in a functional ELISA. Immobilized SLC31A1 at 0.5 μg/well can bind human LGALS8 at7.6293-1000000 ng/mL, the EC₅₀ of human LGALS8 is 14.063-30.957 μg/mL.

Human

E. coli Cell-free

N-SUMO;N-6*His

O15431 (M1-H190)

1317  [NCBI]

MDHSHHMGMSYMDSNSTMQPSHHHPTTSASHSHGGGDSSMMMMPMTFYFGFKNVELLFSGLVINTAGEMAGAFVAVFLLAMFYEGLKIARESLLRKSQVSIRYNSMPVPGPNGTILMETHKTVGQQMLSFPHLLQTVLHIIQVVISYFLMLIFMTYNGYLCIAVAAGAGTGYFLFSWKKAVVVDITEHCH

Approximately 40 kDa & 80 kDa.It is speculated that the protein f orms a dimeric structure 80 kDa.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS,0.05% Brij-78,6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.