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  3. SLC38A1 蛋白, Human (Sf9, His, MBP, FLAG)

SLC38A1 蛋白, Human (Sf9, His, MBP, FLAG)

目录号: HY-P702036
产品使用指南

SLC38A1 蛋白充当同向转运蛋白,促进短链中性氨基酸和钠离子跨细胞膜的 pH 依赖性共转运。至关重要的是,它介导星形胶质细胞衍生的谷氨酰胺转运至 GABA 能中间神经元,有助于 GABA 从头合成。SLC38A1 蛋白, Human (Sf9, His, MBP, FLAG) 是重组的 SLC38A1 蛋白,由 Sf9 insect cells 表达,带有 N-MBP, C-Flag, N-8*His 标签。SLC38A1 蛋白, Human (Sf9, His, MBP, FLAG) 全长 486 个氨基酸。

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描述

The SLC38A1 protein acts as an symporter, facilitating the pH-dependent cotransport of short-chain neutral amino acids and sodium ions across cell membranes. Crucially, it mediates astrocyte-derived glutamine transport to GABAergic interneurons, contributing to de novo GABA synthesis. SLC38A1 Protein, Human (Sf9, His, MBP, FLAG) is the recombinant human-derived SLC38A1 protein, expressed by Sf9 insect cells , with N-MBP, C-Flag, N-8*His labeled tag. The total length of SLC38A1 Protein, Human (Sf9, His, MBP, FLAG) is 486 a.a., .

研究背景

SLC38A1蛋白作为同向转运蛋白发挥作用,促进短链中性氨基酸和钠离子跨细胞膜从细胞外到细胞内的共转运。该生电过程依赖于 pH 值,并由 Na(+) 电化学梯度驱动。值得注意的是,SLC38A1 在介导星形胶质细胞衍生的谷氨酰胺转运至 GABA 能中间神经元中发挥着至关重要的作用,有助于神经递质 GABA 的从头合成。此外,它还参与胎盘滋养层的氨基酸转运。SLC38A1 的调节影响延伸至突触可塑性,强调其参与基本细胞过程,对神经递质合成和氨基酸运输具有影响。

种属

Human

表达系统

Sf9 insect cells

标签

N-MBP;C-Flag;N-8*His

蛋白编号

Q9H2H9 (M2-H487)

基因 ID

81539

蛋白结构
N-term
8*His-MBP
SLC38A1 (M2-H487)
Accession # Q9H2H9
Flag
C-term
同用名
SLC38A1; Sodium-coupled neutral amino acid transporter 1; Amino acid transporter A1; N-system amino acid transporter 2; Solute carrier family 38 member 1; System A amino acid transporter 1; System N amino acid transporter 1
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SLC38A1 Protein, Human (Sf9, His, MBP, FLAG) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SLC38A1 Protein, Human (Sf9, His, MBP, FLAG)
目录号:
HY-P702036
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