SMYD3 蛋白, Human (sf9, GST)

SMYD3 Protein, a histone methyltransferase, specifically induces di- and tri-methylation of H3K4, avoiding monomethylation, and methylates H4K5. Crucial in transcriptional activation within an RNA polymerase complex, SMYD3 also exhibits DNA-binding affinity for sequences containing 5'-CCCTCC-3' or 5'-GAGGGG-3'. SMYD3 Protein, Human (sf9, GST) is the recombinant human-derived SMYD3 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of SMYD3 Protein, Human (sf9, GST) is 335 a.a., with molecular weight of ~58 kDa.

SMYD3 蛋白是一种组蛋白甲基转移酶，在甲基化组蛋白 H3 的“Lys-4”方面表现出特异性，诱导二甲基化和三甲基化，但不诱导单甲基化 (PubMed:15235609、PubMed:22419068)。此外，它还能甲基化组蛋白 H4 的“Lys-5”(PubMed:22419068)。在 RNA 聚合酶复合物中，SMYD3 在转录激活中发挥着至关重要的作用 (PubMed:15235609)。该蛋白质因其能够结合含有 5'-CCCTCC-3' 或 5'-GAGGGG-3' 的 DNA 序列而闻名 (PubMed:15235609)。

Human

Sf9 insect cells

N-GST

Q9H7B4-3 (K35-S369)

64754  [NCBI]

Approximately 58 kDa

Greater than 88% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 0.5 mM DTT, 0.5 mM GSH, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.