SP-10/ACRV1 蛋白, Human (HEK293, C-His)

SP-10/ACRV1 Protein is a testis-specific acrosomal matrix protein, which is evolutionarily conserved among mammals. The gene coding for the SP-10 protein, Acrv1, served as an outstanding model to understand the regulation of male germ-cell-specific gene transcription. The human and mouse SP-10 proteins share homology in the carboxyl terminal region, but contain distinct amino termini. SP-10 probably has an important role in human sperm function, is a promising contraceptive vaccine immunogen for humans, and can serve as a marker protein^[1][2]. SP-10/ACRV1 Protein, Human (HEK293, C-His) is the recombinant human-derived SP-10/ACRV1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of SP-10/ACRV1 Protein, Human (HEK293, C-His) is 244 a.a., with molecular weight of ~35.0 kDa.

SP-10 是一种丰富的蛋白质，存在于顶体基质和顶体内膜中。SP-10 抗体已被证明可用于对小鼠和人类的生精周期进行分期^[1]。
睾丸中有全长 45 kDa SP-10 前体蛋白，并且 32、30、28 和 26 kDa 的 SP-10 肽是由睾丸中 SP-10 前体蛋白的蛋白水解加工和/或选择性拼接产生的^[2]。

Human

HEK293

C-6*His

P26436-1 (Q22-I265)

56  [NCBI]

QPNELSGSIDHQTSVQQLPGEFFSLENPSDAEALYETSSGLNTLSEHGSSEHGSSKHTVAEHTSGEHAESEHASGEPAATEHAEGEHTVGEQPSGEQPSGEHLSGEQPLSELESGEQPSDEQPSGEHGSGEQPSGEQASGEQPSGEHASGEQASGAPISSTSTGTILNCYTCAYMNDQGKCLRGEGTCITQNSQQCMLKKIFEGGKLQFMVQGCENMCPSMNLFSHGTRMQIICCRNQSFCNKI

Approximately 34-36 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.