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  3. SP-17 蛋白, Mouse (His)

SP-17 蛋白, Mouse (His)

目录号: HY-P701070
产品使用指南

SP-17 蛋白作为精子表面透明带结合蛋白,促进精子与透明带的高亲和力结合。它可能与透明带和碳水化合物发生结合相互作用。SP-17 作为同二聚体存在,可能与 ROPN1 相互作用,表明在精卵相互作用和受精过程中发挥合作作用。SP-17 蛋白, Mouse (His) 是重组的 SP-17 蛋白,由 E. coli 表达,带有 N-His 标签。

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描述

SP-17 protein acts as a sperm surface zona pellucida binding protein, facilitating high-affinity spermatozoa binding to the zona pellucida.It potentially engages in binding interactions with zona pellucida and carbohydrates.Existing as a homodimer, SP-17 may interact with ROPN1, suggesting a cooperative role in sperm-egg interactions and fertilization processes.SP-17 Protein, Mouse (His) is the recombinant mouse-derived SP-17 protein, expressed by E.coli , with N-His labeled tag.

研究背景

SP-17蛋白作为精子表面透明带结合蛋白,促进精子与透明带的高亲和力结合。其潜在作用包括参与与透明带和碳水化合物的结合相互作用。SP-17 作为同二聚体存在,也可能与 ROPN1 相互作用,表明在与精卵相互作用和受精相关的过程中可能发挥合作作用。

种属

Mouse

表达系统

E. coli

标签

N-His

蛋白编号

Q62252 (M1-N149)

基因 ID
蛋白结构
N-term
His
SP-17 (M1-N149)
Accession # Q62252
C-term
同用名
Sperm surface protein Sp17; CT22; Sp17-1; Sperm protein 17; SPA17;
分子量

20-25 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SP-17 Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SP-17 Protein, Mouse (His)
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HY-P701070
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