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  3. SPARC 蛋白, Cynomolgus (HEK293, His)

SPARC 蛋白, Cynomolgus (HEK293, His)

目录号: HY-P700830
产品使用指南

SPARC 蛋白参与细胞生长调节,并可能通过影响细胞外基质和细胞因子的相互作用发挥调节作用。SPRC 蛋白能够与钙和铜、几种类型的胶原蛋白、白蛋白、血小板反应蛋白、PDGF 和细胞膜结合。它有两个钙结合位点:其中一个是酸性域,能够以低亲和力结合 5-8 个 Ca2+,另一个是 EF-手环,能以高亲和力结合 Ca2+ 离子。SPARC 蛋白, Cynomolgus (HEK293, His) 是重组的 SPARC 蛋白,由 HEK293 表达,带有 C-His 标签。SPARC 蛋白, Cynomolgus (HEK293, His) 全长 286 个氨基酸,分子量为 43-48 kDa。

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描述

SPARC protein is involved in the regulation of cell growth and may play a regulatory role by affecting the interaction of extracellular matrix and cytokines. SPRC protein can bind calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. It has two calcium binding sites: one is an acidic domain that can bind 5-8 Ca2+ with low affinity, and the other is an EF-hand loop that can bind Ca2+ ions with high affinity. SPARC Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived SPARC protein, expressed by HEK293 , with C-His labeled tag. The total length of SPARC Protein, Cynomolgus (HEK293, His) is 286 a.a., with molecular weight of 43-48 kDa.

研究背景

SPARC 蛋白是富含半胱氨酸的酸性分泌蛋白,又称骨粘连蛋白或 BM-40,是一种调节细胞粘附、细胞外基质产生、生长因子活性和细胞周期的基质细胞蛋白。SPARC 蛋白不发挥结构功能,但具有抗增殖和抗粘附特性,可以调节细胞与周围细胞外基质之间的相互作用。SPRC 蛋白能够与 Ca 和 Cu、几种类型的胶原蛋白、白蛋白、血小板反应蛋白、PDGF 和细胞膜结合。它有两个钙结合位点:其中一个是酸性域,能够以低亲和力结合 5-8 个 Ca2+,另一个是 EF-手环,能以高亲和力结合 Ca2+ 离子。SPARC 在损伤、再生、肥胖、癌症和炎症等部位的过表达,是用于疾病治疗和评估的潜在靶点和治疗指标。

种属

Cynomolgus

表达系统

HEK293

标签

C-His

蛋白编号

G7P8R2 (A18-I303)

基因 ID
蛋白结构
N-term
SPARC (A18-I303)
Accession # G7P8R2
His
C-term
同用名
SPARC; BM-40; ON; EGM_15571
分子量

43-48 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SPARC Protein, Cynomolgus (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SPARC Protein, Cynomolgus (HEK293, His)
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HY-P700830
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