SPARC 蛋白, Rat (HEK293, His)

SPARC protein is involved in the regulation of cell growth and may play a regulatory role by affecting the interaction of extracellular matrix and cytokines. SPRC protein can bind calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. It has two calcium binding sites: one is an acidic domain that can bind 5-8 Ca²⁺ with low affinity, and the other is an EF-hand loop that can bind Ca²⁺ ions with high affinity. SPARC Protein, Rat (HEK293, His) is the recombinant rat-derived SPARC protein, expressed by HEK293 , with C-His labeled tag. The total length of SPARC Protein, Rat (HEK293, His) is 284 a.a., with molecular weight of ~33.8-40 kDa.

SPARC 蛋白是富含半胱氨酸的酸性分泌蛋白，又称骨粘连蛋白或 BM-40，是一种调节细胞粘附、细胞外基质产生、生长因子活性和细胞周期的基质细胞蛋白。SPARC 蛋白不发挥结构功能，但具有抗增殖和抗粘附特性，可以调节细胞与周围细胞外基质之间的相互作用。SPRC 蛋白能够与 Ca 和 Cu、几种类型的胶原蛋白、白蛋白、血小板反应蛋白、PDGF 和细胞膜结合。它有两个钙结合位点：其中一个是酸性域，能够以低亲和力结合 5-8 个 Ca²⁺，另一个是 EF-手环，能以高亲和力结合 Ca²⁺ 离子。SPARC 在损伤、再生、肥胖、癌症和炎症等部位的过表达，是用于疾病治疗和评估的潜在靶点和治疗指标。

Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED₅₀ for this effect is typically 3.581μg/mL, corresponding to a specific activity is 2.793×10² U/mg.

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  Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED₅₀ for this effect is typically 3.581 μg/mL, corresponding to a specific activity is 2.793×10² U/mg.

Rat

HEK293

C-His

P16975 (A18-I301)

24791  [NCBI]

APQTEAAEEMVAEETVVEETGLPVGANPVQVEMGEFEEGAEETVEEVVAENPCQNHHCKHGKVCELDESNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIAPCLDSELTEFPLRMRDWLKNVLVTLYERDEGNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALEEWAGCFGIKEQDINKDLVI

Approximately 33.8-40 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.