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  3. SPARCL1 蛋白, Mouse (283a.a, HEK293, His)

SPARCL1 蛋白, Mouse (283a.a, HEK293, His)

目录号: HY-P76652
COA 产品使用指南

SPARCL1 蛋白具有钙离子、胶原蛋白和细胞外基质结合等重要功能。SPARCL1 主要在谷氨酸能突触膜粘附中发挥作用。SPARCL1 蛋白, Mouse (283a.a, HEK293, His) 是重组的 SPARCL1 蛋白,由 HEK293 表达,带有 N-His 标签。

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规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥680 In-stock
10 μg ¥1080 In-stock
50 μg ¥2800 In-stock
100 μg ¥4500 In-stock
500 μg ¥14000 In-stock
1 mg ¥22400 询价
> 1 mg   询价  

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描述

SPARCL1 protein has important functions such as calcium ion, collagen and extracellular matrix binding. SPARCL1 mainly plays a role in glutamatergic synaptic membrane adhesion. SPARCL1 Protein, Mouse (283a.a, HEK293, His) is the recombinant mouse-derived SPARCL1 protein, expressed by HEK293 , with N-His labeled tag.

研究背景

SPARCL1 蛋白是一种多功能分子,具有钙离子、胶原蛋白和细胞外基质结合活性。它主要定位于谷氨酸能突触,参与突触膜粘附过程。SPARCL1 在多个系统中广泛表达,包括心血管、中枢神经、男性生殖、呼吸和感觉器官系统,尤其在成人膀胱和皮质中高表达,暗示其在这些组织中发挥关键作用。该蛋白在进化上高度保守,其人类同源物 SPARC like 1 的存在进一步证实了其功能重要性。

种属

Mouse

表达系统

HEK293

标签

N-6*His

蛋白编号

NP_034227.3 (Y368-F650)

基因 ID
蛋白结构
N-term
His
SPARCL1 (Y368-F650)
Accession # NP_034227.3
C-term
同用名
SPARC-like protein 1; Extracellular matrix protein 2; Matrix glycoprotein Sc1; ECM2, SC1
氨基酸序列

YLKYGGGEETTTGESENRREAADNQEAKKAESSPNAEPSDEGNSREHSAGSCTNFQCKRGHICKTDPQGKPHCVCQDPETCPPAKILDQACGTDNQTYASSCHLFATKCRLEGTKKGHQLQLDYFGACKSIPACTDFEVAQFPLRMRDWLKNILMQLYEPNPKHGGYLNEKQRSKVKKIYLDEKRLLAGDHPIELLLRDFKKNYHMYVYPVHWQFNELDQHPADRILTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF

分子量

Approximately 38-48 kDa due to the glycosylation.

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

SPARCL1 Protein, Mouse (283a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SPARCL1 Protein, Mouse (283a.a, HEK293, His)
目录号:
HY-P76652
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