SPG3A/ATL1 蛋白, Human (sf9, His)

The SPG3A/ATL1 protein is a GTPase that promotes membrane tethering by forming trans homomers, thereby achieving homotypic fusion of the endoplasmic reticulum (ER) membrane. It makes a crucial contribution to the biogenesis of the endoplasmic reticulum tubular network and shaping the structural organization of cells. SPG3A/ATL1 Protein, Human (sf9, His) is the recombinant human-derived SPG3A/ATL1 protein, expressed by Sf9 insect cells , with N-His labeled tag.

SPG3A/ATL1 蛋白作为 GTP 酶发挥作用，通过形成反式同聚体在膜束缚中发挥关键作用，促进内质网 (ER) 膜的同型融合。它的参与延伸到内质网管状网络的生物发生，有助于这种细胞结构的复杂组织。除了其主要功能外，SPG3A/ATL1 还可能参与高尔基体生物发生和轴突发育的调节。在作为脱辅基蛋白的单体状态和GDP结合形式下，SPG3A/ATL1在与GTP结合时转变为同二聚体，展示了其不同状态之间的动态相互作用。该蛋白质与关键细胞成分（包括 MAP4K4、REEP5、RTN3、RTN4、SPAST、TMED2、REEP1、CPT1C 和 ARL6IP1）进行各种相互作用，突出显示其参与复杂的分子关联网络。此外，SPG3A/ATL1 与 ZFYVE27 相互作用，进一步扩大其监管范围。

Human

Sf9 insect cells

N-10*His

Q8WXF7-1 (M1-T447)

51062  [NCBI]

MAKNRRDRNSWGGFSEKTYEWSSEEEEPVKKAGPVQVLIVKDDHSFELDETALNRILLSEAVRDKEVVAVSVAGAFRKGKSFLMDFMLRYMYNQESVDWVGDYNEPLTGFSWRGGSERETTGIQIWSEIFLINKPDGKKVAVLLMDTQGTFDSQSTLRDSATVFALSTMISSIQVYNLSQNVQEDDLQHLQLFTEYGRLAMEETFLKPFQSLIFLVRDWSFPYEFSYGADGGAKFLEKRLKVSGNQHEELQNVRKHIHSCFTNISCFLLPHPGLKVATNPNFDGKLKEIDDEFIKNLKILIPWLLSPESLDIKEINGNKITCRGLVEYFKAYIKIYQGEELPHPKSMLQATAEANNLAAVATAKDTYNKKMEEICGGDKPFLAPNDLQTKHLQLKEESVKLFRGVKKMGGEEFSRRYLQQLESEIDELYIQYIKHNDSKNIFHAAR

Approximately 54 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 500 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

For concentration less than that, please reconstitute in 50 mM Tris-HCL, 500 mM NaCl, pH 8.0 buffer, 10% Glycerol.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.