SRSF1 蛋白, Human (His-SUMO)

SRSF1 protein plays a crucial role in splicing regulation, preventing exon skipping and ensuring splicing accuracy. It interacts with spliceosomal components, forming a bridge between 5'- and 3'-splice site binding elements. SRSF1 stimulates U1 snRNP binding and prefers purine-rich RNA sequences, functioning as a splicing enhancer. It can act as a splicing repressor through isoforms ASF-2 and ASF-3. Additionally, SRSF1 may facilitate mRNA nuclear export and is identified in the spliceosome C complex, participating in ribonucleoprotein complexes with various RNA-binding proteins. Its interactions with multiple proteins highlight its intricate role in splicing and cellular processes. SRSF1 Protein, Human (His-SUMO) is the recombinant human-derived SRSF1 protein, expressed by E. coli, with N-SUMO, N-6*His labeled tag. The total length of SRSF1 Protein, Human (His-SUMO) is 247 a.a., with molecular weight of 43.6 kDa.

SRSF1 蛋白通过防止外显子跳跃和调节选择性剪接，在维持剪接保真度方面发挥着至关重要的作用。它与剪接体成分的相互作用，在 RS 结构域的促进下，在 5'-和 3'-剪接位点结合元件、U1 snRNP 和 U2AF 之间建立了一座桥梁。SRSF1 优先结合富含嘌呤的 RNA 序列，通过 ASF/SF2 剪接增强子 (ASE) 在体外充当剪接增强子。虽然亚型 ASF-2 和 ASF-3 作为剪接阻遏物发挥作用，但 SRSF1 也可以作为通过 TAP/NXF1 途径的 mRNA 核输出中的输出接头。在剪接体 C 复合体中，它与其他 RNA 结合蛋白合作，包括 DDX5、HNRNPH2 和剪接调节因子 ARVCF。SRSF1 广泛的相互作用组涉及 SAFB/SAFB1、PSIP1/LEDGF、RSRC1、ZRSR2/U2AF1-RS2、CCDC55、SRPK1、NXF1、CCNL1、CCNL2、CDK11B 和 RRP1B 等蛋白质，展示了其在 RNA 加工和细胞功能中的多方面作用。此外，当它在 RS 结构域磷酸化时，它与 TNPO3 的相互作用促进核输入，并且它与 ILDR1 和 ILDR2 相互作用。

Human

E. coli

N-SUMO;N-6*His

Q07955 (S2-T248)

6426  [NCBI]

SGGGVIRGPAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGTGRGGGGGGGGGAPRGRYGPPSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRSRSRSRSRSRSNSRSRSYSPRRSRGSPRYSPRHSRSRSRT

43.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.