ST2/IL1RL1 蛋白, Cynomolgus (HEK293, His)

ST2/IL1RL1 Protein is a member of the Toll-like receptor superfamily, and is a soluble and membrane-bound protein that is the receptor for interleukin 33. It recruits MYD88, IRAK1/4, and TRAF6 after stimulation, leading to MAPK phosphorylation. It is also an inhibitor of interleukin 1 receptor (IL-1RI) and Toll-like receptor 4 (TLR4) signaling, maintaining endotoxin tolerance. ST2/IL1RL1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived ST2/IL1RL1 protein, expressed by HEK293 , with C-His labeled tag.

ST2/IL1RL1 蛋白是 toll 样受体超家族的成员，但与其他成员不同，它不通过激活 NF-κB 来诱导炎症反应，尽管它可以激活 MAP 激酶。ST2/IL1RL1 Protein 具有两种存在形式 (可溶性和膜结合形式)，当心肌被拉伸时，ST2 基因上调，循环可溶性 ST2/IL1RL1 Protein 浓度迅速升高。其膜结合形式作为 IL-33 的受体，与 IL-33 结合可以调节心脏疾病和损伤，如缺血事件，引发心脏保护作用，从而保持心功能。ST2/IL1RL1 Protein 的膜结合形式通过隔离接头 MyD88 和 Mal 来负调控  I型白细胞介素 1 受体 (IL-1RI) 和 Toll 样受体 4 (TLR4) 的信号转导。它可以维持内毒素耐受^[1][2]。
ST2/IL1RL1 Protein 过表达 (OE) 会增加中性粒细胞趋化剂 IL-8 的产生，还增强了HRV1B 诱导的IP-10 (一种参与哮喘恶化的趋化因子)。并且ST2/IL1RL1 Protein 还可促进对气道细菌和病毒感染的促炎反应。因此 ST2/IL1RL1 Protein 在炎症调节中起重要作用^[3]。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Cynomolgus

HEK293

C-His

XP_005575214.1 (M1-C331)

102115135  [NCBI]

Approximately 37.1 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.