STAT1 蛋白, Human (sf9, His-GST)

STAT1 is a signal transducer and activator of transcription that responds to interferons, KITLG/SCF, and growth factors. After type I IFN binds, STAT1 and STAT2 form ISGF3, which translocates to the nucleus and activates ISG transcription. STAT1 Protein, Human (sf9, His-GST) is the recombinant human-derived STAT1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of STAT1 Protein, Human (sf9, His-GST) is 712 a.a., with molecular weight of ~105 kDa.

STAT1 是一种信号转导器和转录激活剂，在介导细胞对干扰素 (IFN)、细胞因子 KITLG/SCF 和各种生长因子的反应中发挥着至关重要的作用。I 型 IFN（IFN-α 和 IFN-β）与细胞表面受体结合后，STAT1 发生酪氨酸磷酸化并与 STAT2 形成二聚体。这种复合物被称为 ISGF3 转录因子，易位到细胞核并激活 IFN 刺激基因 (ISG) 的转录，从而建立抗病毒状态。响应 II 型干扰素 (IFN-gamma)，STAT1 在酪氨酸和丝氨酸残基处被磷酸化，形成同二聚体 (GAF)，进入细胞核并与 IFN-gamma 激活序列 (GAS) 结合，驱动靶标蛋白的表达基因并诱导细胞抗病毒状态。此外，STAT1 响应 KITLG/SCF 和 KIT 信号传导而被激活，并可能介导细胞对激活的 FGFR1、FGFR2、FGFR3 和 FGFR4 的反应。此外，在小肠中，STAT1 与 Gasdermin-D 结合并促进 CIITA 转录，诱导 1 型调节性 T (Tr1) 细胞的形成。STAT1 与多种蛋白质相互作用，包括 PIAS1、IFNAR1、IFNAR2、NMI、CREBBP/CBP、PTK2/FAK1、SRC、ERBB4、PARP9、DTX3L、EP300/p300 和 IFNGR1，有助于其多样化的细胞功能。

Human

Sf9 insect cells

N-His;N-GST

P42224-2 (M1-V712)

6772  [NCBI]

Approximately 105 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.