Syndecan-1/CD138 蛋白, Human (HEK293, His)

Syndecan-1/CD138 Protein, a cell surface proteoglycan, connects the cytoskeleton to the interstitial matrix with heparan sulfate and chondroitin sulfate. It regulates exosome biogenesis with SDCBP and PDCD6IP. Syndecan-1/CD138 Protein induces its own expression through an MSX1 pathway in dental cells and interacts with CDCP1 and TIAM1. It also interacts with MDK, participating in diverse cellular processes. Syndecan-1/CD138 Protein, Human (HEK293, His) is the recombinant human-derived Syndecan-1/CD138 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Syndecan-1/CD138 Protein, Human (HEK293, His) is 234 a.a., with molecular weight of 45-65 kDa.

Syndecan-1/CD138 蛋白是一种细胞表面蛋白聚糖，其特征在于存在硫酸乙酰肝素和硫酸软骨素，使其能够发挥连接细胞骨架与间质基质的作用。它与 SDCBP 和 PDCD6IP 合作调节外泌体生物发生。此外，Syndecan-1/CD138 蛋白不仅能够在牙齿间充质细胞中诱导自身表达，而且还能够通过 MSX1 介导的途径在邻近的牙齿上皮细胞中诱导表达。它与 CDCP1 和 TIAM1 相互作用，特别是分别通过其 C 端和 TIAM1 的 PDZ 域。此外，Syndecan-1/CD138 蛋白与 MDK 相互作用，促进其参与各种细胞过程。

Measured by the ability of the immobilized protein to enhance the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin. The ED₅₀ for this effect is 1.555 μg/mL in the presence of 0.5 μg/mL Fibronectin, corresponding to a specific activity is 643.087 units/mg.

Human

HEK293

C-6*His

P18827 (Q18-E251)

6382  [NCBI]

QPALPQIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHLASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKE

Approximately 43-77 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.