T4 ligase 蛋白, T4 phage (His)

The T4 ligase protein is expressed during the early stages of lytic development and serves as a DNA ligase involved in various aspects of T4 phage biology. It plays a crucial role in T4 DNA synthesis, promoting the formation of phosphodiester bonds to join DNA fragments. T4 ligase Protein, T4 phage (His) is the recombinant T4 ligase protein, expressed by E. coli , with N-6*His labeled tag. T4 ligase Protein, T4 phage (His), has molecular weight of ~55.3 kDa.

T4 连接酶是一种 DNA 连接酶，在 T4 噬菌体生命周期的裂解发育早期阶段表达。该酶是 T4 DNA 合成和基因重组过程中不可或缺的一部分，参与 DNA 片段的连接。除了在 DNA 合成和重组中的作用之外，T4 连接酶还参与 T4 DNA 修复机制，表明它参与维持病毒基因组的完整性。T4 连接酶的多功能性质强调了它在与 T4 噬菌体发育相关的复杂过程中的重要性，并强调了它在确保病毒 DNA 保真度和稳定性方面的作用（参考通道不可用）。

Others

E. coli

N-6*His

P00970

1258680  [NCBI]

MILKILNEIASIGSTKQKQAILEKNKDNELLKRVYRLTYSRGLQYYIKKWPKPGIATQSFGMLTLTDMLDFIEFTLATRKLTGNAAIEELTGYITDGKKDDVEVLRRVMMRDLECGASVSIANKVWPGLIPEQPQMLASSYDEKGINKNIKFPAFAQLKADGARCFAEVRGDELDDVRLLSRAGNEYLGLDLLKEELIKMTAEARQIHPEGVLIDGELVYHEQVKKEPEGLDFLFDAYPENSKAKEFAEVAESRTASNGIANKSLKGTISEKEAQCMKFQVWDYVPLVEIYSLPAFRLKYDVRFSKLEQMTSGYDKVILIENQVVNNLDEAKVIYKKYIDQGLEGIILKNIDGLWENARSKNLYKFKEVIDVDLKIVGIYPHRKDPTKAGGFILESECGKIKVNAGSGLKDKAGVKSHELDRTRIMENQNYYIGKILECECNGWLKSDGRTDYVKLFLPIAIRLREDKTKANTFEDVFGDFHEVTGL

Approximately 55.3 kDa

Greater than 95% as determined by reducing SDS-PAGE.