TAOK3 蛋白, Human (sf9, His)

TAOK3, a kinase, regulates the p38/MAPK14 and MAPK8/JNK cascades. It activates the p38/MAPK14 cascade, particularly in DNA damage-induced G2/M transition, likely through MAP2K3 and MAP2K6 phosphorylation. TAOK3 inhibits basal MAPK8/JNK activity and dampens its response to epidermal growth factor. These functions showcase TAOK3's role in coordinating cellular responses to DNA damage and growth factor signaling. TAOK3 Protein, Human (sf9, His) is the recombinant human-derived TAOK3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of TAOK3 Protein, Human (sf9, His) is 411 a.a..

TAOK3 是一种丝氨酸/苏氨酸蛋白激酶，作为 p38/MAPK14 应激激活的 MAPK 级联和 MAPK8/JNK 级联的调节器。它作为 p38/MAPK14 级联的激活剂，特别是在响应 DNA 损伤时，通过激活 p38/MAPK14 级联在 G2/M 转变 DNA 损伤检查点中发挥作用。这种激活可能是通过上游 MAP2K3 和 MAP2K6 激酶的磷酸化介导的。此外，TAOK3 作为 MAPK8/JNK 级联基础活性的抑制剂，并减少其响应表皮生长因子 (EGF) 的激活。这些调节功能凸显了 TAOK3 在协调细胞对 DNA 损伤和生长因子信号传导反应中的作用。

Measured by its ability to catalyze 250 μg/mL PKCtide peptide substrate adenosine and 25 μM ATP that incubate at room temperature for 40 minutes. The specific activity is 32.49 nmo/min/mg.

Human

Sf9 insect cells

N-10*His

Q9H2K8 (M1-D411)

51347  [NCBI]

MRKGVLKDPEIADLFYKDDPEELFIGLHEIGHGSFGAVYFATNAHTSEVVAIKKMSYSGKQTHEKWQDILKEVKFLRQLKHPNTIEYKGCYLKEHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAITHGALHGLAYLHSHALIHRDIKAGNILLTEPGQVKLADFGSASMASPANSFVGTPYWMAPEVILAMDEGQYDGKVDIWSLGITCIELAERKPPLFNMNAMSALYHIAQNDSPTLQSNEWTDSFRRFVDYCLQKIPQERPTSAELLRHDFVRRDRPLRVLIDLIQRTKDAVRELDNLQYRKMKKILFQETRNGPLNESQEDEEDSEHGTSLNREMDSLGSNHSIPSMSVSTGSQSSSVNSMQEVMDESSSELVMMHDDESTINSSSSVVHKKDHVFIRDEAGHGD

Approximately 54 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 500 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.