TCblR/CD320 蛋白, Mouse (HEK293, His)

TCblR/CD320 Protein, the receptor for cobalamin-saturated transcobalamin (TCbl), plays a pivotal role in cobalamin uptake, expressed on the plasma membrane and notably on follicular dendritic cells (FDC). As a costimulator, TCblR promotes B cell responses, fostering differentiation and proliferation. It's particularly influential in differentiating germinal center-B cells, engaging in collaborative interactions with T-cells and FDC. CD320 enhances IL-10-induced precursor proliferation, underscoring its significance in cobalamin homeostasis and cellular processes through interactions with TCN2. TCblR/CD320 Protein, Mouse (HEK293, His) is the recombinant mouse-derived TCblR/CD320 protein, expressed by HEK293, with C-His labeled tag. The total length of TCblR/CD320 Protein, Mouse (HEK293, His) is 208 a.a., with molecular weight of ~47.7 kDa.

TCblR/CD320 蛋白作为钴胺素饱和转钴胺素 (TCbl) 的受体，在钴胺素摄取中发挥着关键作用。它位于质膜上，主要在滤泡树突细胞 (FDC) 上表达，促进与生发中心 B 细胞的相互作用。TCblR 作为共刺激剂，促进 B 细胞对抗原刺激的反应，从而促进 B 细胞分化和增殖。TCblR 对生发中心 B (GC-B) 细胞向记忆 B 细胞和浆细胞 (PC) 的分化特别有影响，它与 T 细胞和滤泡树突细胞 (FDC) 协同相互作用。它的作用延伸到增强 IL-10 产生的 PC 前体的增殖。CD320 与 TCN2 的相互作用通过其 LDL 受体 A 类结构域介导，强调了其在钴胺素稳态和细胞过程中的重要性。

Measured by its binding ability in a functional ELISA. Immobilized mouse CD320-His at 10 μg/mL (100 μl/well) can bind biotinylated mouse TCN2-His , The EC₅₀ of biotinylated mouse TCN2-His is 0.10-0.24 μg/mL.

Mouse

HEK293

C-His

Q9Z1P5-1 (M1-G208)

54219  [NCBI]

MARGGAGRAVALGLVLRLLFGLRTGLEAAPAPAHTRVQVSGSRADSCPTDTFQCLTSGYCVPLSWRCDGDQDCSDGSDEEDCRIESCAQNGQCQPQSALPCSCDNISGCSDVSDKNLNCSRPPCQESELHCILDDVCIPHTWRCDGHPDCLDSSDELSCDTDTEIDKIFQEENATTTRISTTMENETSFRNVTFTSAGDSSRNPSAYG

Approximately 47.7 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.