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TCEB3 蛋白, Human (Sf9)

目录号: HY-P701515
产品使用指南

TCEB3,也称为 SIII,作为关键的转录延伸因子,促进 RNA 聚合酶 II 进展超出阻断位点。它与SIII调节亚基B和C相互作用形成elongin BC复合物,显着增强亚基A的转录活性。TCEB3 蛋白, Human (Sf9) 是重组的 TCEB3 蛋白,由 Sf9 insect cells 表达,不带标签。TCEB3 蛋白, Human (Sf9) 全长 797 个氨基酸。

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描述

TCEB3, also known as SIII, acts as a key transcription elongation factor that promotes RNA polymerase II progression beyond the blocking site. It interacts with SIII regulatory subunits B and C to form the elongin BC complex, which significantly enhances the transcriptional activity of subunit A. TCEB3 Protein, Human (Sf9) is the recombinant human-derived TCEB3 protein, expressed by Sf9 insect cells , with tag free. The total length of TCEB3 Protein, Human (Sf9) is 797 a.a., .

研究背景

TCEB3,也称为 SIII,作为通用转录延伸因子发挥作用,促进 RNA 聚合酶 II 转录延伸超出模板编码的阻止位点。亚基 A 的转录活性通过其与 SIII 调节亚基 B 和 C 形成的二聚体复合物(统称为 elongin BC 复合物)的相互作用而得到显着增强。TCEB3 在多亚基组装体中运作,包括 elongin BC 复合物(ELOB 和 ELOC)、elongin A/ELOA、RBX1 和 CUL5。在这种情况下,它对单泛素化 POLR2A 进行多泛素化,有助于转录过程的调节。

种属

Human

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

Q14241 (H2-R798)

基因 ID

6924

蛋白结构
N-term
TCEB3 (H2-R798)
Accession # Q14241
C-term
同用名
ELOA (TCEB3); Elongin-A; EloA; Elongin 110 kDa subunit; RNA polymerase II transcription factor SIII subunit A1; SIII p110; Transcription elongation factor B polypeptide 3
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TCEB3 Protein, Human (Sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TCEB3 Protein, Human (Sf9)
目录号:
HY-P701515
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