TCN1 蛋白, Human (P.pastoris, His)

The TCN1 protein is critical for mitochondrial processes and binds to the mitochondrial light chain promoter to regulate mitochondrial transcription. As a key component of the mitochondrial transcription initiation complex (including TFB2M and POLRMT), TCN1 plays a crucial role in the basal transcription of mitochondrial DNA. TCN1 Protein, Human (P.pastoris, His) is the recombinant human-derived TCN1 protein, expressed by P. pastoris , with N-His labeled tag. The total length of TCN1 Protein, Human (P.pastoris, His) is 410 a.a., with molecular weight of ~47.6 kDa.

TFAM 是线粒体过程的关键参与者，具有与线粒体轻链启动子结合的能力，从而有助于线粒体转录的调节。作为线粒体转录起始复合物（包括 TFB2M、TFAM 和 POLRMT）的组成部分，它在线粒体 DNA 的基础转录中发挥着至关重要的作用。在这个复合体中，TFAM 不仅将 POLRMT 招募到特定的启动子上，而且还与 TFB2M 合作诱导 POLRMT 的结构变化，促进启动子打开和捕获 DNA 非模板链。TFAM 对于线粒体 RNA 聚合酶准确识别启动子至关重要，可促进选定启动子的转录起始。此外，TFAM 还展示了 DNA 解旋能力，并通过其 HMG 盒为线粒体轻链启动子 DNA 赋予 U 形转弯形状。除了转录功能外，TFAM 对于维持正常的线粒体 DNA 水平至关重要，并且可能在组织和压缩线粒体和核 DNA 中发挥作用，特别是在精子发生过程中。

Human

P. pastoris

N-His

P20061 (24E-433Y)

6947  [NCBI]

EICEVSEENYIRLKPLLNTMIQSNYNRGTSAVNVVLSLKLVGIQIQTLMQKMIQQIKYNVKSRLSDVSSGELALIILALGVCRNAEENLIYDYHLIDKLENKFQAEIENMEAHNGTPLTNYYQLSLDVLALCLFNGNYSTAEVVNHFTPENKNYYFGSQFSVDTGAMAVLALTCVKKSLINGQIKADEGSLKNISIYTKSLVEKILSEKKENGLIGNTFSTGEAMQALFVSSDYYNENDWNCQQTLNTVLTEISQGAFSNPNAAAQVLPALMGKTFLDINKDSSCVSASGNFNISADEPITVTPPDSQSYISVNYSVRINETYFTNVTVLNGSVFLSVMEKAQKMNDTIFGFTMEERSWGPYITCIQGLCANNNDRTYWELLSGGEPLSQGAGSYVVRNGENLEVRWSKY

Approximately 47.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.