TEAD4 蛋白, Human (His)

TEAD4 protein is a transcription factor that plays a key role in the Hippo signaling pathway and is essential for organ size control and tumor suppression. It coordinates proliferation inhibition and apoptosis promotion through a kinase cascade involving MST1/MST2, SAV1, LATS1/2, and MOB1. TEAD4 Protein, Human (His) is the recombinant human-derived TEAD4 protein, expressed by E. coli , with N-6*His labeled tag. The total length of TEAD4 Protein, Human (His) is 361 a.a., with molecular weight of ~44.7 kDa.

TEAD4 是一种转录因子，在 Hippo 信号通路中发挥着关键作用，Hippo 信号通路是一个通过协调增殖抑制和促进细胞凋亡来控制器官大小和抑制肿瘤至关重要的调节网络。该通路的核心特征是激酶级联，其中 MST1/MST2 与其调节蛋白 SAV1 一起磷酸化并激活与其调节伙伴 MOB1 复合的 LATS1/2。MOB1 随后磷酸化并灭活 YAP1 癌蛋白和 WWTR1/TAZ。TEAD4 通过介导 YAP1 和 WWTR1/TAZ 的基因表达发挥作用，从而监督细胞过程，例如增殖、迁移和上皮间质转化 (EMT) 诱导。该蛋白表现出与 Sph 和 GT-IIC“enhansons”(5'-GTGGAATGT-3') 的特异性和非合作性结合，激活转录，并且还与 M-CAT 基序相互作用。TEAD4 与 YAP1 和 WWTR1/TAZ 的复杂相互作用强调了它在该信号通路的调节动态中的核心作用。

Human

E. coli

N-6*His

Q15561 (74M-434E)

7004  [NCBI]

MYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARRKAREIQAKLKDQAAKDKALQSMAAMSSAQIISATAFHSSMALARGPGRPAVSGFWQGALPGQAGTSHDVKPFSQQTYAVQPPLPLPGFESPAGPAPSPSAPPAPPWQGRSVASSKLWMLEFSAFLEQQQDPDTYNKHLFVHIGQSSPSYSDPYLEAVDIRQIYDKFPEKKGGLKDLFERGPSNAFFLVKFWADLNTNIEDEGSSFYGVSSQYESPENMIITCSTKVCSFGKQVVEKVETEYARYENGHYSYRIHRSPLCEYMINFIHKLKHLPEKYMMNSVLENFTILQVVTNRDTQETLLCIAYVFEVSASEHGAQHHIYRLVKE

Approximately 44.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HC1, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.