2. 重组蛋白
  3. Biotinylated Proteins
  4. TFPI 蛋白, Human (Biotinylated, HEK293, His-Avi)

TFPI 蛋白, Human (Biotinylated, HEK293, His-Avi)

目录号: HY-P78212
产品使用指南

TFPI（组织因子途径抑制剂）直接抑制因子 X (Xa)，并通过 Xa 依赖性机制抑制 VIIa/组织因子的活性。它可能形成涉及 Xa、TFPI、VIIa 和组织因子的四元复合物。TFPI 具有抗血栓特性，并且可以与脂蛋白结合，证明其在调节凝血途径中的多方面作用。TFPI 蛋白, Human (Biotinylated, HEK293, His-Avi) 是重组的 TFPI 蛋白，由 HEK293 表达，带有 C-Avi, C-His 标签。TFPI 蛋白, Human (Biotinylated, HEK293, His-Avi) 全长 254 个氨基酸，分子量为 40-50 kDa。

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描述

TFPI (Tissue Factor Pathway Inhibitor) directly inhibits factor X (Xa) and inhibits the activity of VIIa/tissue factor through an Xa-dependent mechanism. It potentially forms a quaternary complex involving Xa, TFPI, VIIa, and tissue factor. TFPI exhibits antithrombotic properties and can associate with lipoproteins, demonstrating its multifaceted role in regulating the coagulation pathway. TFPI Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived TFPI protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of TFPI Protein, Human (Biotinylated, HEK293, His-Avi) is 254 a.a., with molecular weight of 40-50 kDa.
研究背景

TFPI（组织因子途径抑制剂）直接抑制因子 X (Xa)，并以 Xa 依赖性方式抑制 VIIa/组织因子的活性，可能是通过形成涉及 Xa、TFPI、VIIa 和组织因子的四元复合物。这种蛋白质具有抗血栓特性，并且能够与血浆中的脂蛋白结合，显示出其在调节凝血途径中的多方面作用。
种属

Human
表达系统

HEK293
标签

C-Avi;C-His
蛋白编号

P10646 (D29-K282)
基因 ID
蛋白结构
N-term
TFPI (D29-K282)
Accession # P10646
His-Avi
C-term
同用名
LACI; TFPI1; EPI; TFI; convertin; TFPI
分子量

40-50 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

TFPI Protein, Human (Biotinylated, HEK293, His-Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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目录号：
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