TGF alpha/TGFA 蛋白, Human (CHO)

TGF-alpha Protein, Human (CHO) is a potent angiogenic mediator and mitogenic polypeptide.

转化生长因子（TGF）是一种多肽，可以赋予几种正常细胞表型转化。TGF-α 与表皮生长因子 (EGF) 受体结合，已从多种肿瘤细胞中分离出来。TGF-α 在早期胎儿发育期间的胚胎、多种病毒转化细胞以及多种人类肿瘤中合成^[1]。TGF-α 是有丝分裂剂表皮生长因子家族的成员，与人类结肠癌细胞转化特性的进展相关^[2]。

The ED₅₀ is <0.4 ng/mL as measured by 3T3 cells.

Human

CHO

Tag Free

P01135 (V40-A89)

7039  [NCBI]

VVSHFNDCPDSHTQFCFHGTCRFLVQEDKPACVCHSGYVGARCEHADLLA

8-10 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Schreiber AB, et al. Transforming growth factor-alpha: a more potent angiogenic mediator than epidermal growth factor. Science. 1986 Jun 6;232(4755):1250-3.  [Content Brief]

  [2]. DuBois RN, et al. Regulation of eicosanoid production and mitogenesis in rat intestinal epithelial cells by transforming growth factor-alpha and phorbol ester. J Clin Invest. 1994 Feb;93(2):493-8.  [Content Brief]