TGF alpha/TGFA 蛋白, Mouse (HEK293, Fc)

TGF α/TGFA is a mitogenic peptide that binds to the EGF receptor (EGFR) and acts synergistically with TGF β to achieve anchorage-independent cell proliferation in soft agar.It interacts with SDCBP and the SNTA1 PDZ domain, which is critical for cell surface localization.TGF alpha/TGFA Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived TGF alpha/TGFA protein, expressed by HEK293 , with N-hFc labeled tag.

TGF α 是一种促有丝分裂多肽，具有与 EGF 受体 (EGFR) 结合的能力，并与 TGF β 协同作用，促进软琼脂中非贴壁依赖性细胞增殖。它与 SDCBP 和 SNTA1 的 PDZ 结构域相互作用，前者对于其定位到细胞表面至关重要。TGF α 以其未成熟形式存在于内质网中，其特征是前片段和部分 N-糖基化，可与 CNIH 相互作用。在高尔基体内，它可能与 CNIH 和 GORASP2 形成复合物。此外，TGF α 通过其细胞质 C 末端结构域与 NKD2 以及 MAGI3 相互作用。

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.2054 ng/mL.

Mouse

HEK293

N-hFc

P48030 (V39-A88)

21802  [NCBI]

38-45 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.