TGF beta 1/TGFB1 LAP 蛋白, Human (HEK293, N-His)

TGF beta 1 is a secreted ligand of the TGF-beta superfamily that binds to receptors and activates SMAD transcription factors. Preproprotein is proteolytically processed to produce latency-associated peptide (LAP) and mature peptide. TGF beta 1/TGFB1 LAP Protein, Human (HEK293, N-His) is the recombinant human-derived TGF beta 1/TGFB1 Latency-associated peptide protein, expressed by HEK293 , with N-6*His labeled tag and C33S mutation.

TGF beta 1 蛋白是该基因的产物，编码属于 TGF-beta 超家族的分泌配体，以其在调节各种细胞过程中的作用而闻名。作为配体，它与不同的 TGF-β 受体结合，启动控制基因表达的 SMAD 家族转录因子的招募和激活。编码的前蛋白原经过蛋白水解加工，产生潜伏相关肽 (LAP) 和成熟肽。它以包含成熟肽同二聚体、LAP同二聚体和潜在TGF-β结合蛋白的潜在形式存在，或者以仅由成熟肽同二聚体组成的活性形式存在。这种蛋白可以与其他 TGFB 家族成员形成异二聚体，在调节细胞增殖、分化和生长中发挥着关键作用。此外，它还能够调节其他生长因子的表达和激活，包括干扰素 γ 和肿瘤坏死因子 α。该基因的突变在肿瘤细胞中经常上调，导致卡穆拉蒂-恩格尔曼病。它的表达在多种组织中广泛观察到，在脾脏 (RPKM 38.0)、骨髓 (RPKM 32.1) 和 23 个其他组织中表达水平显着，凸显了它广泛参与生理过程。

Measured by its ability to inhibit TGF-beta 1 activity on HT‑2 mouse T cells. The ED ₅₀ for this effect is 55.73 ng/mL in the presence of 1 ng/mL of Recombinant Human TGF beta 1,corresponding to a specific activity is 1.79×10^4 units/mg.

Human

HEK293

N-6*His

P01137/NP_000651.3 (L30-R278, C33S)

7040  [NCBI]

LSTSKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRR

37-44 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.