TGF beta 1/TGFB1 蛋白, Mouse/Rat (HEK293)

TGF beta 1/TGFB1 Protein, Mouse/Rat (HEK293), is a recombinant cytokine produced in HEK293 cells, is implicated as a key regulator of the development and cyclic remodelling characteristic of reproductive tissues^[1][2].

TGFB1（转化生长因子β1）是一种有效的细胞因子，在发育、纤维化和癌症中发挥驱动作用。它被合成为前结构域-生长因子复合物，需要与 LTBP（潜在转化生长因子 β 结合蛋白）结合才能有效分泌到细胞外空间。释放后，这种大型潜在复合物通过锚定到细胞外基质 (ECM) 网络而被隔离，成熟的生长因子需要从该网络被激活才能到达其受体并启动信号传导^[2]。

The ability to inhibit IL-4-dependent proliferation of TF-1 human erythroleukemic cells has an ED50 value of 5-25 pg/mL.

Mouse; Rat

HEK293

Tag Free

P04202(A279-S390)

21803  [NCBI]

ALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Approximately 13 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 4 mM HCl.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 4mM HCl. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Ingman WV, et al. The essential roles of TGFB1 in reproduction. Cytokine Growth Factor Rev. 2009;20(3):233-239.  [Content Brief]

  [2]. Nüchel J, et al. TGFB1 is secreted through an unconventional pathway dependent on the autophagic machinery and cytoskeletal regulators. Autophagy. 2018;14(3):465-486.  [Content Brief]